高血压大鼠心脏肌凝蛋白重链mRNA表达和卡托普利的作用  

Expression of cardiac myosin heavy chains mRNA in hypertensive rats and effect of captopril

作　　者：陈义汉[1,2,3,4] 龚兰生[1,2,3,4] 陆林 茅矛[1,2,3,4] 陈赛娟 

机构地区：[1]上海铁道大学医学院附属甘泉医院心脏内科 [2]上海第二医科大学附属瑞金医院心脏内科 [3]上海市血液研究所 [4]卫生部人类基因组重点实验室

出　　处：《中华心血管病杂志》1997年第6期423-425,共3页Chinese Journal of Cardiology

摘　　要：目的：观察自发性高血压大鼠（ＳＨＲ）心肌肥厚时心脏α和β－肌凝蛋白重链（α－ＭＨＣ和β－ＭＨＣ）ｍＲＮＡ表达和卡托普利的保护作用。方法：１５周龄雄性ＳＨＲ口服卡托普利（１００ｍｇｋｇ－１／ｄ）１２周（ＣＡＰ组），同样年龄性别的ＳＨＲ（ＳＨＲ组）和Ｗｉｓｔａｒ－ｋｙｏｔｏ大鼠（ＷＫＹ组）不给药，在同样条件下喂养同样时间，通过称重法计算左室重量指数（ＬＶＩ），使用测微技术测量心肌细胞横径（ＴＤＭ），采用Ｎｏｒｔｈｅｒｎ印迹技术检测左室α和β－ＭＨＣｍＲＮＡ水平。结果：（１）ＳＨＲ组ＬＶＩ和ＴＤＭ显著高于ＷＫＹ组；ＣＡＰ组上述指标显著低于ＳＨＲ组，而与ＷＫＹ组无显著差异；（２）ＳＨＲ组α－ＭＨＣｍＲＮＡ表达明显受抑，β－ＭＨＣｍＲＮＡ表达明显增加；卡托普利抑制α－ＭＨＣｍＲＮＡ水平降低，但不影响β－ＭＨＣｍＲＮＡ水平。结论：高血压肥厚心肌ＭＨＣ基因出现了异常表达，卡托普利可以防止这种现象的发生。The aim of the study was to observe expression of cardiac α and βmyosin heavy chains (αMHC and βMHC) mRNA in hyp ertrophied hearts of spontaneously hypertensive rats (SHR) and protective effect of captopril. 15weekold male SHRs were treated with oral captopril (100mg·kg-1/d) for 12 weeks (CAP group), and age and sexmatched SHRs (SHR group) and WistarKyoto rats (WKY group) were fed under the same conditions but without captopril administration for the same duration. By weighing weight, left ventricular weight indices (LVI) were calculated. Using micrometry, transverse diameters of myocytes (TDM) were measured. With Northern blot technique, levels of αMHC mRNA and βMHC mRNA of left ventricles were determined. The results showed that:(1) LVI and TDM in SHR group were significantly higher than those in WKY group; all the above parameters in CAP group were significantly lower than those in SHR group, but they were not significantly different from those in WKY group;(2)Expression of αMHC mRNA was apparently inhibited, but expression of βMHC mRNA was apparently increased in SHR group; captopril inhibited the decrease of αMHC mRNA level, but did not affect βMHC mRNA level. It is concluded that there is abnormal expression of cardiac MHC genes in hypertrophied heart in hypertension and captopril can prevent the abnormal phenomenon.

关 键 词：高血压 心脏 肌凝蛋白重链 MRNA 卡托普利 

分 类 号：R544.1[医药卫生—心血管疾病]