LC-MS-MS分析烟草中氨基甲酸酯农药残留  

Determination of Carbamates Residues in Tobacco by LC-MS-MS

作　　者：龚炜[1,2] 刘惠民[2] 石杰[1] 谢复炜[2] 王昇[2] 

机构地区：[1]郑州大学化学系,河南省郑州市450052 [2]中国烟草总公司郑州烟草研究院,河南省郑州450001

出　　处：《烟草科技》2008年第8期44-48,共5页Tobacco Science & Technology

基　　金：河南省科技攻关项目(0496430006);河南省教育厅自然科学基础研究项目(0411021200)

摘　　要：为建立烟草样品中11种氨基甲酸酯农药残留的分析方法，采用液相色谱-质谱／质谱（LC-MS-MS）联用分析技术，使用丙酮／水溶液振荡提取烟草中的农药残留成分，萃取液经过二氯甲烷液液分配，然后采用固相萃取柱（NH2）净化。使用反相液相色谱对农残样品进行分离，以氘代甲萘威为内标，采用多反应监控模式（MRM）进行定量分析。结果表明，当加标质量浓度水平为0．05、0．2、0．4μg／g时，平均加标回收率为69．2％-121．0％，相对标准偏差为1．0％-12．0％。In order to develop a method for the determination of residues of 11 carbamate pesticides in tobacco, liquid chromatography-mass/mass spectrometry was applied. Pesticide residues were extracted with acetone/water, the extract was liquid-liquid distributed by dichloromethane and cleaned up by NH2 column. The samples were separated by reversed-phase liquid chromatography, and the eluent was determined by LC-MS-MS in the multiple reaction monitoring mode, and with D7-carbaryl as an internal standard. The results showed that ： when the mass concentrations of added standards were 0.05, 0.2, 0.4μg/g, the average recovery was from 69. 2% to 121.0% and the relative standard deviation was from 1.0% to 12.0%. It satisfied the requirements of multi-pesticide residue analysis.

关 键 词：氨基甲酸酯 农药残留 液相色谱-质谱/质谱 

分 类 号：S481.8[农业科学—农药学]