西瓜中类胡萝卜素的柱色谱法分离分析  

Analysis of Carotenoids in Watermelon by Column Chromatography Separation

作　　者：贾关荣[1] 赵文恩[1] 申红山[1] 王斌[1] 马双武[2] 

机构地区：[1]郑州大学化学工程学院,河南郑州450001 [2]中国农业科学院郑州果树研究所,河南郑州450009

出　　处：《安徽农业科学》2008年第20期8416-8418,8501,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]采用柱色谱法分析检测西瓜中的类胡萝卜素。[方法]以5种瓤色品种的西瓜为试材,通过开放柱色谱法对西瓜主要类胡萝卜素进行分离分析。[结果]红、黄、桃红、粉与白瓤瓜5种西瓜都含有六氢番茄红素、β-和ζ-胡萝卜素。除白瓤瓜外,其他颜色的瓜瓤都含有番茄红素,以红瓤瓜的含量最多,早花、红一号与96B41的番茄红素含量分别为35.35、28.44和21.19 mg/kg。黄瓤西瓜307超风的β-胡萝卜素含量约0.48 mg/kg,白瓤西瓜三白的ζ-胡萝卜素含量约0.4 mg/kg。红色与桃红色瓜瓤中分离检测到γ-胡萝卜素,红色与黄色瓤瓜中分离检测到叶黄素类。[结论]开放柱色谱法具有成本低、操作简便、结果可靠的特点,可用于分析常量样品中类胡萝卜素各组分含量。[Objective] The research aimed to analyze and detect the carotenoids from watermelon by column chromatography.[Method] The main carotenoids were separated and analyzed by open column chromatography from five watermelon cultivars with different flesh color [Result] Five watermelon cultivars with red-,yellow-,coral-,pink-and white-flesh colors all contained phytofluene,β-,and ζ-carotene.Apart from white-flesh watermelons,the other color flesh cultivars contained lycopene,with its content in red-flesh watermelon being the most.The lycopene content in early flowering,Red 1 and 96B41 was 35.35,28.44 and 21.19 mg/kg resp.β-carotene content in yellow-flesh watermelon 307Chaofeng was about 0.48 mg/kg,and ζ-carotene content in white-flesh watermelon Three white was about 0.4 mg/kg.γ-carotene could be separated and detected in the red-and coral-flesh cultivars.Lutein could be separated and detected in the red-and yellow-flesh cultivars.[Conclusion] Open column chromatography had the characteristic of low cost,e asy operation and reliable results so it can be used to analyze the lycopene content in each component in constant samples.

关 键 词：西瓜 类胡萝卜素 开放柱色谱法 

分 类 号：O657.7[理学—分析化学]