1株拟盘多毛孢菌的培养·纯化及其ITS区的克隆测序  

Cultivation,Purification,ITS Region Cloning and Sequencing of a Strain of Pestalotiopsis spp.

作　　者：邹立扣[1] 潘欣[2] 张翅[3] 

机构地区：[1]四川农业大学都江堰分校生物科学系,四川都江堰611830 [2]成都理工大学地球科学学院园林系,四川成都610059 [3]四川农业大学林学院,四川雅安625014

出　　处：《安徽农业科学》2008年第20期8507-8509,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省教育厅青年基金项目(2006B014);四川农业大学青年创新基金;成都理工大学青年基金

摘　　要：[目的]为拟盘多毛孢菌分离、纯化及ITS区克隆测序相关研究提供依据。[方法]对鸡枞菌内生拟盘多毛孢菌进行分离、纯化,并通过形态学及分子生物学的方法进行鉴定,对其ITS区进行PCR扩增和克隆测序分析。[结果]形态学鉴定结果显示没有已报道的拟盘多毛孢菌与其相似,系统发育树结果也表明其属于一单独分支中。[结论]建立拟盘多毛孢菌的ITS序列数据库是十分必要的。[Objective] The research aimed to provide references for the study of Pestalotiopsis spp.isolation,purification and ITS region cloning and sequencing.[Method] The endogenous Pestalotiopsis spp.in Termitomyces albuminosus was isolated,purified and identified by the means of morphology and molecular biology.Pestalotiopsis spp.ITS region was amplified with PCR and analyzed after cloning and sequencing.[Result] Morphology identification demonstrated that there was no other similar Pestalotiopsis spp.with which isolated from the study.And sequence alignment analysis revealed it belonged to single branch.[Conclusion] It was quite necessary to establish the ITS sequence database of Pestalotiopsis spp.

关 键 词：培养 拟盘多毛孢属 克隆 

分 类 号：S182[农业科学—农业基础科学]