沟叶结缕草组织培养表面灭菌方法的筛选  

Screening of Surface Sterilization Methods in the Tissue Culture of Zoysia matrella

作　　者：王栋[1] 买合木提.克衣木 玉永雄[1] 胡艳[1] 

机构地区：[1]西南大学动物科技学院重庆市牧草与草食家畜重点实验室重庆市高校草食动物工程研究中心 [2]新疆巴州动物疾病控制与诊断中心,新疆巴音郭楞蒙古自治州841000

出　　处：《安徽农业科学》2008年第20期8560-8561,共2页Journal of Anhui Agricultural Sciences

基　　金：国家重点基础研究发展计划(2007CB108901)资助

摘　　要：[目的]为建立沟叶结缕草再生体系奠定基础。[方法]以沟叶结缕草带芽茎段为外植体,通过正交试验研究0.1%升汞、5%次氯酸钠、75%酒精和预处理方法等因素对外植体成活率的影响,筛选最佳表面灭菌方法。[结果]各因素对外植体成活率的影响顺序为0.1%升汞>5%次氯酸钠>75%酒精>预处理方法,而0.1%升汞和5%次氯酸钠对其影响分别达到极显著和显著水平。沟叶结缕草组织培养的最佳表面灭菌方法为:先用5 g洗洁精加水冲洗2次,再使用5%次氯酸钠(滴入3滴100%吐温80),振荡灭菌10 min;最后用0.1%升汞(滴入3滴100%吐温80),振荡灭菌10 min。[结论]可用杀菌剂、抗生素等对外植体进行预处理或将抗生素加入培养基中,起到杀菌和抑菌的作用。[Objective] The research aimed to lay the foundation for establishing the regeneration system of Zoysia matrelia.[Method] With stem with buds of Zoysia matrella as explants,the effects of such factors as 0.1%mercuric chloride,5%sodium hypochlorite,75%alcohol and pretreatment methods on the survival rate of explants were studied though the orthogonal test.And the optimum surface sterilization methods was screened out.[Result] The effects of each factor on the survival rate of explants orderly were 0.1%mercuric chloride 〉5%sodium hypochlorite 〉75%alcohol 〉 pretreatment methods and the effects of 0.1%mercuric chloride and 5%sodium hypochlorite reached at extremely significant level and at a significant level.The optimum sterilization method in the tissue culture of Zoysia matrella was as follows： flushing with 5 g detergent and water for 2 times, and sterilizing with 5%sodium hypochlorite（dropping 3 drops of 100%Tween 80） for 10 min under vibratory conditions and finally sterilizing with 0.1%mercuric chloride （dropping 3 drops of 100%Tween 80） for 10 min under vibratory conditions.[Conclusion] It could play the role of sterilization and bacteriostasis to pretreat explants with bactericide,antibiotic and so on or adding antibiotic to the medium.

关 键 词：正交试验设计 表面灭菌方法 沟叶结缕草 

分 类 号：S688.4[农业科学—观赏园艺]