红叶李离体茎段培养与微体快繁的研究  

Study on In Vitro Stem Culture and Rapid Propagation of Prunus cerasifera Ehrh.cv.Atropurpurea

作　　者：刘长春 廖林正 雷光祥 刘奕清 

机构地区：[1]重庆高校园林花卉工程研究中心,重庆400041 [2]重庆铁路中心,重庆永川402168

出　　处：《安徽农业科学》2008年第21期8910-8911,8965,共3页Journal of Anhui Agricultural Sciences

基　　金：重庆市教委重大平台建设项目(GCZX0713);重庆文理学院重点科研项目

摘　　要：[目的]为红叶李的商品化生产、遗传转化和品种定向改良奠定基础。[方法]以红叶李茎段为外植体,在MS、1/2MS基本培养基中添加不同浓度的6-BA和NAA,研究不同激素浓度对红叶李增殖和生根的影响,建立红叶李的离体繁殖体系。[结果]适量浓度的6-BA和NAA组合比单独使用6-BA诱导率高,处理⑤(6-BA、NAA的浓度分别为1.0、0.2mg/L)的诱导率达75.0%,且腋芽生长良好。处理⑥(6-BA、NAA的浓度分别为2.0、0.2mg/L)的增殖系数为7.67,芽苗高度大于2cm的芽数为4.76。适合红叶李生根的最佳IBA浓度为0.5mg/L,生根率达98.3%。试管苗移栽成活率达85%以上。[结论]红叶李离体培养的最佳启动培养基为MS+6-6-BA1.0mg/L+NAA0.2mg/L,最佳增殖培养基为MS+6-BA2.0mg/L+NAA0.2mg/L,最佳生根培养基为1/2MS+IBA0.5mg/L。[Objective] The study aimed to lay the base for the commercial production,genetic transformation and variety-directed improvement of Prunus cerasifera Ehrh.cv.Atropurpurea.[Method] With the stems of P.cerasifera as the explants,6-BA and NAA with different concn.were added into MS and 1/2 MS medium to study the effect of different hormone concn.on the proliferation and rooting of P.cerasifera and establish the in vitro propagation system of P.cerasifera.[Result] The induction rate in the combination of 6-BA,NAA and NAA with proper concn.was higher than that in the treatment with single 6-BA.T5 with 6-BA and NAA at 1.0 and 0.2 mg/L resp.could get the induction of 75.0% and make axilla buds grow well.T6 with 6-BA and NAA at 2.0 and 0.2 mg/L resp.could get the proliferation coefficient of 7.67 and had buds of 4.76 whose height was over 2 cm.The optimum IBA concn,suiatable for rooting of P.cerasifera was 0.5 mg/L,with rooting of 98.3%.The survival rate for transplanting of tube seedlings was over 85%.[Conclusion] The best initial medium for in vitro culture of P.cerasifera was MS＋6-BA1.0 mg/L＋NAA 0.2 mg/L,the best medium for proliferation was MS＋6-BA 2.0 mg/L＋NAA＋0.2 mg/L and the best medium for rooting was 1/2 MS＋IBA 0.5 mg/L.

关 键 词：红叶李 离体培养 快速繁殖 

分 类 号：S722.37[农业科学—林木遗传育种]