侧柏叶总黄酮的提取工艺研究  

Research on the Best Extraction Technique of Flavonoids from Platycladus orientalis Leaf

作　　者：赵莹[1] 张建平[1] 赵永光[1] 周永国[1] 

机构地区：[1]河北科技师范学院化学系,河北秦皇岛066004

出　　处：《安徽农业科学》2008年第21期9103-9104,共2页Journal of Anhui Agricultural Sciences

基　　金：河北省科技厅科技攻关项目部分内容

摘　　要：[目的]探讨提取侧柏叶总黄酮的最优工艺。[方法]采用索氏提取法,选用不同溶剂在不同的浸提时间、浸提温度、液料比下对侧柏叶总黄酮提取工艺进行研究。[结果]最优浸提方案是浸提时间6h、浸提温度70℃、液料比8∶1;浸提温度对提取侧柏叶总黄酮的影响较大,其次为浸提时间,而液料比的影响较小;甲醇作提取剂的效果最佳。按最优提取工艺得总黄酮产率为8.450%。定性试验和紫外光谱分析结果表明,侧柏叶中的黄酮化合物主要为黄酮醇类。[结论]该研究提出了侧柏叶总黄酮的最优提取工艺,为侧柏叶总黄酮的提取奠定了基础。[Objective]The extraction technique of flavonoids from Platycladus orientalis leaf was researched with the different solvents,extraction times,temperatures and the proportion of solvent material.The result of orthogonal test： the flavonoids extracted from Platycladus orientalis leaf was affected by the extraction temperature,time and solvent material proportion with degression order.The best extraction technique scheme was the solvent was methanol;the extraction time,6 h;the extraction temperature,70 ℃ and solvent material proportion,8∶1.The result of the best extraction technique scheme was flavone content was 8.450%.

关 键 词：侧柏叶 总黄酮 提取 

分 类 号：Q943.1[生物学—植物学]