DGGE技术在动物胃肠道微生态研究中的应用  

Application of DDGE technique to study animal gastrointestinal microbiota

作　　者：李彩燕[1] 王伟[2] 

机构地区：[1]浙江大学动物科学学院 [2]南京农业大学动物科技学院

出　　处：《中国饲料》2008年第14期14-17,共4页China Feed

基　　金：国家973项目"畜禽肉品质性状形成的营养代谢与调控机理"(2004CB117506)

摘　　要：变性梯度凝胶电泳(Denaturing Gradient Gel Electrophoresis,DGGE)技术是通过直接分离核酸片段对微生物群落进行研究,由于其具有可靠性强、重复性好、方便快捷等优点,被广泛应用于微生物群落多样性和动态性分析,并成为微生物分子生态学研究中的热点技术之一。本文主要介绍DGGE技术的原理、工作流程及优缺点,并着重分析了其在动物胃肠道微生态研究中的应用。Denaturing gradient gel electrophoresis（DGGE）is based on direct separate of nucleic acid and has the advantages of reliability, recurrence and facility.It is a well-established molecular tool in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities.DGGE has become one of the most widely used methods of intestinal microflora research,The principle, workflow ,pros and cons of DGGE were discussed in this review.In addition, the applications of DGGE technique to study animal gastrointestinal microbiota were highly addressed.

关 键 词：变性梯度凝胶电泳 胃肠道微生态 

分 类 号：S811.6[农业科学—畜牧学]