无花果叶超氧化物歧化酶的分离、纯化及性质研究  

Separation,Purification and Properties of Superoxide Dismutase from Ficus carica Leaves

作　　者：张雷[1] 曲和之[1] 黄露[1] 杜姗姗[1] 郝东云[1] 王晓平[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130021

出　　处：《高等学校化学学报》2008年第8期1588-1591,共4页Chemical Journal of Chinese Universities

摘　　要：以我国山东无花果树(Brunswike,原产法国Ficus carica)树叶为原料,采用缓冲液抽提、硫酸铵分级、DEAE-Sepharose Fast Flow阴离子交换层析和Sephacryl S-100HR分子筛层析分离纯化得到电泳纯CuZn-SOD,并对其酶学性质进行研究.A CuZn-SOD from Ficus carica leaves was separated and purified with ammonium sulfate fractionation, DEAE-Sepharose fast flow chromatography and Sephacryl S-100 HR chromatography techniques. One major bright band was achieved on polyacrylamide gel electrophoresis stained with nitroblue tetrazolium （NBT）. The molecular weight of the enzyme subunit was determined by SDS-PAGE to be about 17800. We investigated the physical and chemical properties of the enzyme, and characterized its kinetic properties, such as the optimal pH, optimal temperature and the effect of chemical reagents andmetal ions on the SOD. The results show that the stability of this purified SOD appeared to be better than other SODs reported. The analysis results of circular dichroism spectrum reveals that the enzyme possessed approximate 12.78% of α-helix in its secondary structure.

关 键 词：无花果树树叶 超氧化物歧化酶 分离纯化 

分 类 号：Q554[生物学—生物化学]