超亲疏水图案改善阵列芯片质量  

Improving Quality of Microarray Chip Based on Superhydrophilic-Superhydrophobic Patterned Surface

作　　者：阴启明[1] 叶嘉明[1] 周勇亮[1] 

机构地区：[1]厦门大学化学化工学院化学系,化学生物学福建省重点实验室,厦门361005

出　　处：《高等学校化学学报》2008年第8期1647-1649,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20675066);中国科学院有机固体重点实验室开放基金资助

摘　　要：Microarray chip including genechip has been of great application in fields of biology, quarantine, pharmacy and so on. Most of microarray chips were prepared by spotting aqueous droplet on the surface of substrate. Due to 'coffee-ring stain effect' , there always was a denser perimeter where sample was concentrated during the process of droplet drying after spotting. So the ununiformity of spot caused by 'coffee-ring stain effect' was one troublesome problem of the chip quality. Herein, superhydrophilic spot arrays on superhydrophobic AAO surface were fabricated to ease the 'coffee-ring stain effect' . Furthermore, a protein array is prepared on this patterned surface where immunoreactions are carried out. As a result, spot uniformity is greatly improved in a wide range of concentration and volume.Microarray chip including genechip has been of great application in fields of biology, quarantine, pharmacy and so on. Most of microarray chips were prepared by spotting aqueous droplet on the surface of substrate. Due to ＂coffee-ring stain effect＂, there always was a denser perimeter where sample was concentrated during the process of droplet drying after spotting. So the ununiformity of spot caused by ＂coffee-ring stain effect＂ was one troublesome problem of the chip quality. Herein, superhydrophilie spot arrays on superhydrophobic AAO surface were fabricated to ease the ＂coffee-ring stain effect＂. Furthermore, a protein array is prepared on this patterned surface where immunoreactions are carried out. As a result, spot uniformity is greatly improved in a wide range of concentration and volume.

关 键 词：阵列芯片 咖啡环效应 超亲水 超疏水 图案化 

分 类 号：O647[理学—物理化学]