急性心肌梗死后囊性纤维化跨膜转运调节体氯通道基因表达的变化  

Expression of cystic fibrosis transmembrane conductance regulator gene induced by acute myocardial infarction

作　　者：刘育[1] 黄从新[1] 唐艳红[1] 袁明杰[1] 

机构地区：[1]武汉大学人民医院心内科,430060

出　　处：《实用医学杂志》2008年第16期2751-2753,共3页The Journal of Practical Medicine

基　　金：湖北省"十一五"重大科技攻关项目(编号:2006AA301A04)

摘　　要：目的:研究猪急性心肌梗死(AMI)后囊性纤维化跨膜转运调节体(CFTR)氯通道基因表达的变化,探讨急性心肌梗死后早期室性心律失常发生的分子机制。方法:通过结扎猪左前降支远端1/3～1/2处2h然后再灌注建立AMI模型(AMI组),同时设立相应的假手术组。术后24h取左心室梗死区、边缘区和正常区内层、中层和外层心肌(假手术组取对应位置心肌),应用逆转录-聚合酶链反应(RT-PCR)半定量分析CFTR氯通道基因表达的改变。结果:与假手术组相比,AMI组CFTRmRNA表达在梗死区三层心肌中均明显下降(P<0.05),在边缘区三层心肌中均明显上升(P<0.05),在正常区三层心肌中均没有显著性改变(P>0.05)。AMI组CFTRmRNA表达在梗死区和边缘区的三层心肌之间以及在梗死区、边缘区和正常区同一层心肌之间差异有显著性(P<0.05)。结论:AMI后CFTR氯通道基因表达在梗死区、边缘区和正常区心肌之间以及局部心肌三层间呈不均一性改变,可能是AMI后室性心律失常发生的分子生物学基础之一。Objective To investigate the changes of the expression of cystic fibrosis transmembrane conductance regulator （CFTR） gene induced by acute myocardial infarction （AMI） in pigs. Methods The pig model of A MI was established by ligating left anterior descending coronary artery for 2 h followed with reperfusion. 24 h after surgery, semiquantitative reverse transcriptase-polymerase chain reaction （RT-PCR） was used to measure the CFTR mRNA in each group. Results Compared with the sham-group, CFTR mRNA expression was significantly down-regulated in all three layers of infract zone（P 〈 0.05） ,but significantly up-regulated in all three layers of border zone（P 〈 0.05） of AMI group, and no significant change of CFTR mRNA expression was detected among three myocardium layers of normal zone （P 〉 0.05）. In AMI group, the CFTR mRNA expression was significantly different among three myocardium layers of infarct zone or border zone, and in different zones of the same myocardium layer （P 〈 0.05）. Conclusion The heterogeneous expression of CFTR gene in different zones and myocardium layers may be one of the molecular mechanisms involved in ventricular arrhvthmias induced by AMI.

关 键 词：心肌梗塞 心律失常 心血管病学 氯通道 囊性纤维化跨膜转运调节体 

分 类 号：R542.22[医药卫生—心血管疾病]