肝舒汤防治大鼠非酒精性脂肪性肝病的实验研究  

Preventative and Therapeutic Effects of Ganshutang Decoction on Non-alcoholic Fatty Liver Disease in Experimental Rats

作　　者：杨成志[1] 狄灵[1] 聂丹丽[1] 于燕[2] 阮班云[1] 

机构地区：[1]西安交通大学医学院第二附属医院中西医结合科,陕西省西安市710004 [2]西安交通大学医学院

出　　处：《中国全科医学》2008年第17期1550-1553,共4页Chinese General Practice

基　　金：陕西省科技攻关资助项目〔No.2005K16-G5(4)〕

摘　　要：目的探讨中药肝舒汤防治非酒精性脂肪性肝病的作用及机制。方法健康雄性SD大鼠32只,随机分为正常组、模型组、中药组、东宝肝泰组各8只。除正常组喂食普通饲料外,其余各组均喂食高脂饲料大鼠非酒精性脂肪性肝病模型。正常组、模型组:灌服1ml/100g0.9%氯化钠溶液;东宝肝泰组:灌服5g/L的羧甲基纤维素钠配制成的东宝肝泰混悬液;中药组:灌服肝舒汤药液。于干预8周后HE染色观察肝脏病理改变,检测血脂、肝匀浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及血清肿瘤坏死因子(TNF-α)含量。结果4组大鼠炎症活动度计分间差异有统计学意义(P<0.05),且模型组与正常组、中药组间差异有统计学意义(P<0.05)。4组大鼠TG、LDL-C、HDL-C间差异均有统计学意义(P<0.05),中药组与模型组TG、LDL-C间差异均有统计学意义(P<0.05);4组大鼠肝匀浆MDA、SOD间差异均有统计学意义(P<0.05),中药组和东宝肝泰组与模型组MDA含量间差异均有统计学意义(P<0.05),而SOD活性间差异无统计学意义(P>0.05)。中药组与模型组干预4周、8周后血清TNF-α的含量间差异均有统计学意义(P<0.05)。结论肝舒汤对大鼠非酒精性脂肪性肝病有明显的防治作用,其作用机制与其改善肝脏组织脂肪变性程度、降低血脂、抑制脂质过氧化、减轻由于脂质过氧化所带来的肝损害、降低血清TNF-α的含量、减少炎性细胞的浸润有关。Objective To observe the effects of Chinese herbs on lipid peroxidation reaction in rats with non - alcoholic fatty liver （ NAFL）, to inquire into its prevention and treatment mechanism. Methods Thirty - two male SD rats were randomly allocated into 4 groups： control group, model group, Dongbao Gantai （DBG） group and Ganshutang decoction （GSTD） group, 8 in each. All groups except the control were fed with an 8 - week high fat diet to prepare NAFL models. General condition, pathological changes of liver tissues were observed, and blood lipids and the level of tumor necrosis factor - alpha （ TNF - α） were detected, so were the levels of MDA, SOD of liver tissues. Results In model group, teatosis appeared in liver tissues which were gradually infiltrated with inflammation cells, and necrosis was present in hepatocyte, percolate in portal area. There was significant difference in the scores of inflammation activity between model and control groups （ P 〈 0. 05）, model group was significantly different from control and GSTD groups （ P 〈 0. 05）, and there was significant difference between DBG and GSTD groups （ P 〈 0. 05）. There was significant difference in TG, LDL - C, HDL - C between 4 groups （ P 〈 0. 05 ）, so was there between model and control groups （ P 〈 0. 05）, and significant difference was seen in levels of TG, LDL - C between DBG and model groups （ P 〈 0. 05 ） ; There was significant difference in serum levels of TG, LDL - C, HDL - C between DBG and model groups （ P 〈 0. 05） and there was significant difference in serum levels of LDL - C, HDL - C between DBG and GSTD groups （ P 〈 0. 05）. In model group, the levels of TNF - α, MDA in liver tissues obviously increased, while the active levels of SOD obviously decreased. As comparing with model group, GSTD could improve the liver pathology structure, and play a role in decreasing significantly blood lipids, the level of TNF - α and liver tissue MDA. Conclusion Ganshutang decoction can prevent a

关 键 词：脂肪肝 肝舒汤 大鼠 中药疗法 

分 类 号：R285.5[医药卫生—中药学]