人工牛黄鉴定的可行性研究  

Study on the Feasibility for identification of Artificial Bezoar

作　　者：曹建业 

机构地区：[1]衡东县人民医院,湖南衡阳421400

出　　处：《中医药导报》2008年第8期115-117,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:检验人工牛黄鉴定方法的可行性。方法:采用薄层色谱法对胆酸、猪去氧胆酸、贝斯素成分进行色谱鉴别,紫外-可见分光光度法测定胆酸、胆红素的含量。结果:鉴别方法专属性强、稳定性良好。含量测定胆酸为14.1%,胆红素为0.72%,准确度高。结论:用薄层色谱法鉴别人工牛黄中成分,结果明显、稳定。分光光度法测定含量准确度高,操作简便、快速,是较为理想的方法。Objective： To check the feasibility of for identification of artificial bezoar. Methods： Thin-layer chromatography method was used to identify the cholic acid, doxicholic acid of pig. The content of cholic acid and bilirubin were tested by ultraviolet and visible spectrophotometry. Results：The method is of good specificity and stability, with high accuracy. The content of cholic acid was 14.1%, and that of acid bilirubin was 0.72%. Conclusion： The results that thin-layer chromatography for identification of the components in artificial bezoar is obvious and stable, Ultraviolet and visible spectrophotometry has high accuracy, easy manipulation to test the content.

关 键 词：人工牛黄 胆酸 贝斯素 胆红素 紫外-可见分光光度法 

分 类 号：R927[医药卫生—药学]