丹皮药材及其伪品、混淆品核糖体rDNA ITS2的序列分析  

Sequences analyses of rDNA-ITS2 of Paeonia suffruticosa Andr and its counterfeit

作　　者：刘海兵[1] 宋社吾[2] 刘道芳[3] 宋文成[1] 冯艺戎[2] 宋礼华[2] 

机构地区：[1]安徽大学生命科学学院,合肥230039 [2]安徽省生物研究所,合肥230088 [3]安徽安科生物工程股份有限公司,合肥230088

出　　处：《生物学杂志》2008年第4期33-36,共4页Journal of Biology

基　　金：安徽省自然科学基金(03043101);安徽省优秀青年科技基金(04043056)

摘　　要：分析安徽道地药材丹皮(Paeonia suffruticosa Andr)及其伪品、混淆品核糖体DNA第二内转录间隔区(ITS2)的序列。分析丹皮的分子遗传多样性,为丹皮的真品药材基原鉴定提供分子依据。采用DNA克隆测序技术对从不同市场购得的丹皮样品及其伪品、混淆品的ITS2基因进行测序分析研究。丹皮与其伪品川赤芍、亳州芍药的rDNA-ITS2序列长度均为412bp。排序比较发现不同市场上的丹皮样品rDNA-ITS2序列变异位点丰富。鉴定出与丹皮真品(黄沙与白沙)及伪品的rDNA-ITS2序列完全相同的样品。其他样品的rDNA-ITS2序列与丹皮真品的差异明显。通过丹皮ITS2序列差异比较分析。ITS2序列可以作为鉴别丹皮药材真伪的一种手段。rDNA-ITS2 sequence of Paeonia suffruticosa Andr and its counterfeit was analyzed. Genetic diversity of Paeonia suffruticosa Andr was studied rDNA-ITS2 sequence for the Paeonia suffruticosa Andr and its counterfeit was sequenced. The sequence length of Paeonia suffruticosa Andr and its counterfeit, is 412bp. There are plenty of variable sites in pairwise comparison of the sequence of Paeonia suffruticosa Andr. rDNA-ITS2 sequence is a method in identifying Paeonia suffruticosa Andr and its counterfeit.

关 键 词：牡丹皮 ITS2序列 DNA测序 

分 类 号：Q523.8[生物学—生物化学]