紫叶酢浆草体细胞胚诱导和发育条件研究  

Study on inducing condition of somatic embryogenesis and embryoid growth of Oxalis traingularis ASt. Hil.

作　　者：李耀亭[1] 陈存武[1] 

机构地区：[1]皖西学院化学与生命科学系,安徽六安237012

出　　处：《生物学杂志》2008年第4期55-57,共3页Journal of Biology

基　　金：皖西学院自然科学基金青年项目;项目编号:WXZQ0411

摘　　要：通过组织培养方法,探讨生长素和细胞分裂素在紫叶酢浆草体细胞胚诱导和发育过程中的作用。结果表明0.5mg/LNAA是诱导胚性愈伤组织最适宜的生长素浓度,添加一定比例的6-BA可有效促进体细胞胚的发生;在附加激素浓度分别低于0.1mg/LNAA和0.5mg/L6-BA的MS培养基上,体细胞胚能发育成完整的植株。因此,一定浓度的生长素是诱导紫叶酢浆草胚性愈伤组织的关键因素,细胞分裂素在体细胞胚的发生和发育过程中起增效作用。The effects of auxins and cytokinins on the process of somatic embryo genesis and embryoid growth of Oxalis traingularis A. St. Hil. by Tissue culture were studied. The results exclaimed that 0. 5 mg/L NAA was the best concentration to induce embryonic callus. Certain ratio 6-BA will cooperate in the process of somatic embryo genesis. The plant would be regenerated when the somatic embryoid was transferred into the MS basal medium without plant hormones or with 0. 2 mg/L NAA and 0.5 mg/L 6-BA. So a certain concentration of auxins was important to the induction of embryonic callus. Cytokinins were cooperative in the process of somatic embryo genesis and embryoid growth.

关 键 词：紫叶酢浆草 组织培养 体细胞胚 

分 类 号：Q943.1[生物学—植物学]