反相高效液相色谱法测定补骨脂酊中补骨脂素和异补骨脂素的含量  

Determination of psoralen and isopsoralen in Buguzhi tincture by RP-HPLC

作　　者：徐梅[1] 邵珠民[1] 吕冬梅[1] 韩强[1] 

机构地区：[1]徐州医学院附属医院药剂科,江苏徐州221002

出　　处：《徐州医学院学报》2008年第8期542-545,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的建立反相高效液相色谱法(RP-HPLC)测定补骨脂酊中补骨脂素(psoralen)和异补骨脂素(isopsoralen)的含量。方法以Waters XTerra RP18(4.6mm×250mm,5μm)为色谱柱,以乙腈-水(0.28∶0.52)为流动相,检测波长247nm。结果补骨脂素和异补骨脂素保留时间分别为14.326min和15.259min;补骨脂素的线性范围为0.88～14.08mg·L-1(r=0.9998);异补骨脂素的线性范围为0.80～12.80mg·L-1(r=0.9999)。补骨脂素的平均加样回收率为99.5%,RSD为1.10%;异补骨脂素的平均加样回收率为100.4%,RSD为0.59%。结论本方法简便、快速、准确,可用于补骨脂酊制剂的质量控制。Objective An RP - HPLC method was established for determination of psoralen and isopsoralen in Bu- guzhi tincture. Methods The XTerra RP18 （4.6 mm ×250mm,5μm） was used. The mobile phase consisted of acetonitrile - water （0.28 ： 0.52 ）. The detection wave length was 247 nm. Results The retention time of psoralen and isopsoralen angelicin was 14. 326 min and 15. 259 rain respectively. The calibration range of psoralen was 0.88 - 14.08 mg·L^-1. （r =0.9998） and that of isopsoralen was 0.80 -12.80 mg·L^-1 （r =0. 9999） ; the average recovery was 99.5% （ RSD = 1.10% ） and 100.4% （ RSD = 0.59% ） respectively. Conclusion The method, being simple, rapid and accurate, can be used to examine the quality of Buguzhi tincture.

关 键 词：补骨脂酊 补骨脂素 异补骨脂素 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]