松仁多糖化学结构的初步分析  

Preliminary Study on Chemical Structure of Pine Nut Kernel Polysaccharide

作　　者：刘荣[1] 孙芳 陈秀丽[1] 王向宏 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]内蒙古纺织工业学校,内蒙古赤峰024000 [3]黑龙江省自然资源研究所,黑龙江哈尔滨150040

出　　处：《林产化学与工业》2008年第4期115-117,共3页Chemistry and Industry of Forest Products

基　　金：黑龙江省科学技术专项资金(QC07C58)

摘　　要：以松仁为原料,经热水提取、乙醇沉淀、DE-52纤维素柱层析和Sephadex G-100柱层析分离得到4个多糖组分(PNP1-a、PNP1-b、PNP2-a及PNP3-a)。经GPC法测定,其相对分子质量依次为10.2×104、1.43×104、11.0×104和16.3×104。其中,PNP1-a由鼠李糖和葡萄糖组成,物质的量之比为1.00∶2.57;PNP1-b由阿拉伯糖和葡萄糖组成,物质的量之比为1.00∶4.03;PNP2-a和PNP3-a均由葡萄糖一种单糖组成。经酸水解、红外光谱(FT-IR)、紫外光谱(UV)和核磁共振光谱(NMR)对各组分进行结构分析,确定4种多糖均为β-构型的吡喃糖。Four polysaccharide fractions （ PNP1-a, PNP1-b, PNP2-a, PNP3-a） were isolated from the kernel of Pinus koraiensis Sieb. et Zucc by hot water extraction, ethanol precipitation and purification followed by DEAE-cellulose and gel-permeation column chromatographies. The molecular weights of the 4 fractions were 10.2 × 10^4, 1.43× 10^4, 11.0× 10^4 and 16.3 × 10^4 respectively. The unit monosaccharides of PNPI-a were identified as rhamnose and glucose at the ratio of 1.00 ： 2.57 ; and the unit monosaccharide of PNPI-b was identified as arabinose and glucose at the ratio of 1.00 ： 4.03 ; PNP2-a and PNP3-a were both consisted of glucose. The structures of the fractions were analysed through FT-IR, ultraviolet and NMR spectroscopies, the results showed that monosaccharides of PNP1-a, PNP1-b, PNP2-a and PNP3-a were all of β-pyranose configuration.

关 键 词：松仁多糖 结构 性质 

分 类 号：TQ91[化学工程]