β-环糊精选择性提取虎杖化学成分及体外抗内毒素活性研究  

Study on Selective Abstraction of Chemical Constituents from Polygonum Cuspidatum with β-cyclodextrin and Activity of Antiendotoxin in Vitro

作　　者：马坤芳[1] 王德旺[2] 任勇[3] 

机构地区：[1]南京医科大学药学院,江苏南京210029 [2]南京医科大学第一附属医院药剂科,江苏南京210029 [3]南京师范大学新药研究中心,江苏南京210097

出　　处：《上海中医药杂志》2008年第8期81-82,共2页Shanghai Journal of Traditional Chinese Medicine

摘　　要：目的研究β-环糊精选择性提取虎杖化学成分及其体外抗内毒素活性。方法利用水溶液中β-环糊精与客体分子包合结晶法,选择提取虎杖中的化学成分;LOPS法考察包合提取部位抗内毒素活性。结果β-环糊精选择性分离出虎杖中的化学成分并形成了包合物晶体,差热扫描图谱确证了包合物的形成;该包合物的体外抗内毒素作用强于阳性对照多粘菌素B。结论β-环糊精选择性提取部位活性明确,无需分离可直接用于制剂。Objective To study selective abstraction of chemical constituents from Chinese Herb Polygonum Cuspidatum with β-cyclodextrin and activity of antiendotoxin in vitro. Methods Chemical constituents of Polygonum Cuspidatum was selectively abstracted by crystallization of inclusion complex of object molecule with β-cyclodextrin in aqueous solution antiendotoxin activity of abstraction was investigated by LOPS way. Results The complex crystal was formed by selective abstraction of chemical constituent of Polyganum Cuspidatum with β- cyclodextrin; inclusion complex was identified by DTA spectroscopy：in antiendotoxin, the inclusion complex was superior to polymyxin B. Conclusion Chemical constituents of Polygonum Cuspidatum selectively abstracted with β-cyclodextrin is accurate,and can be directly used for pharmaceutical preparation.

关 键 词：环糊精 虎杖 化学成分 选择性提取 抗内毒素活性 

分 类 号：R284.2[医药卫生—中药学]