葛根异黄酮的抗氧化作用  

Antioxidation of Pueraria Lobata Isoflavones

作　　者：张光成[1] 方思鸣[1] 

机构地区：[1]同济医科大学基础医学院生物学教研室,武汉430030

出　　处：《同济医科大学学报》1997年第5期340-343,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金!No.39070962

摘　　要：通过葛根异黄酮对动物组织中的脂质过氧化产物（LPO）含量和超氧化物歧化酶（SOD）活性进行研究，探讨葛根异黄酮类化合物的抗自由基作用。体外实验表明，葛根异黄酮0．01～1.0g／L可明显抑制小鼠肝、肾组织及大白兔脑组织匀浆在振荡温育条件下引起的LPO升高，并有剂量效应关系。在1．0g／L的剂量时，对小鼠肝、肾及兔脑组织匀浆中LPO的抑制率分别达79．7％、84．7％和86．6％。体内实验表明，免经液氮冷冻致脑损伤脑水肿后，其血清及脑组织中的SOD活性明显降低，LPO含量明显升高。通过静脉注射不同剂量的葛根异黄酮，在不同时间测定其血中及脑组织中SOD活性及LPO含量，结果表明，葛根异黄酮对降低脑水肿动物血、脑组织中LPO含量有明显作用，而且对提高动物血、脑组织中SOD活性有极显著作用。By studying the effect of pueraia lobata isoflavones (PLIs) on lipid peroxide (LPO) and superoxide dismutase (SOD) in animal tissue, this paper suggests that PLIs is an effective antioxidant. The results in vitro showed that PLIs could inhibit significantly the content of LPO. The highest inhibition rates were found to be 79.7%, 84.7% and 86.6% in liver, kidney of mice and brain of rabbit respectively. In the case of cold induced brain injury with chalcone, the content of LPO and activity of SOD in animal blood and brain were changed. When anima1s were injected with PLIs, the result indicated that the PLIs could reduce significant1y the content of LPO and enhance the activity of SOD in animal blood and brain, being in a dose-dependent relationship.

关 键 词：葛根 葛根异黄酮 LPO SOD 抗氧化作用 

分 类 号：R282.710.5[医药卫生—中药学]