链脲佐菌素诱导的糖尿病大鼠胃底腺中壁细胞的体视学研究  

The Stereoligical Study on Parietal Cells in Oxyntic Glands of Streptozotocin-induced Diabetic Rats

作　　者：赵超[1] 王庆堂[1] 秦光明 李肇春[1] 张敏海[1] 张亦农[1] 汪薇曦 

机构地区：[1]同济医科大学基础医学院组织胚胎学教研室,武汉430030

出　　处：《同济医科大学学报》1997年第5期348-350,共3页Acta Universitatis Medicinae Tongji

摘　　要：采用HE染色显示壁细胞，结合生物体视学技术对链脲佐菌素诱导的糖尿病大鼠在胃底腺中的壁细胞进行了研究。结果显示：糖尿病状态的早期，胃底腺腺体上部壁细胞的体密度显著增加，而数密度却明显减少；腺体下部壁细胞的体密度和面密度均无显著变化，但数密度却显著地减少。提示糖尿病状态下，壁细胞数量显著减少，但体积却明显增大。根据正常情况下壁细胞的细胞动力学变化及上述实验结果，认为大鼠胃底腺峡部的干细胞向壁细胞分化成熟的功能在胰岛素缺乏的情况下受到一定程度的抑制，而这种功能的抑制可能是糖尿病状态下易出现目粘膜萎缩和胃酸分泌减少的重要原因之一。The stereological survey on parietal cells in the gastric oxyntic glands of the streptozotocin-induced diabetic rats was investegated using combined HE staining to display the parietal cells and Weibel's stereological methods. The results showed that the volume density of parietal cells in upper oxyntic glands was increased significant1y in diabetic circumstances, but the numerical density was reduced obviously; and that both volume density and surface density of the parietal cells in lower glands had no significant difference between controls and diabetes. However, the numerical density of the parietal cells in lower oxyntic gland was very significantly reduced. The above results revealed that the volume of parietal cells was significantly increased, but the number of these cells was significantly reduced in the diabetic circumstance. According to the parietal cell's kinetics under normal circumstances and the effects of streptozotocin-induced diabetes on parietal cell's stereological data, we suspect that the function of granule-free cells or stem cells in isthmus of oxgutic glands to trans form and maturate into fully differentiated parietal cells might be partly inhibited because of the deficiency of insulin. And the inhibition of differential function of the granule-free cells might be one of the important pathogenetic mechnisms of diabetes mellitus with atrophic gastritis and decrease of gastric acid secretion.

关 键 词：链脲菌素 糖尿病 壁细胞 体视学 胃 

分 类 号：R587.1[医药卫生—内分泌] R322.4[医药卫生—内科学]