地高辛标记探针与生物素标记探针用于斑点杂交检测HBV DNA的初步研究  

PRELIMINARY STUDY ON THE DETECTION OF HBV DNA BY DOT HYBRIDIZATION WITH DIG-LABELLED OR BIOTIN-LABELLED PROBES

作　　者：郭大为[1] 

机构地区：[1]泸州医学院附属医院,646000

出　　处：《泸州医学院学报》1997年第2期94-97,共4页Journal of Luzhou Medical College

摘　　要：为研究地高辛标记和生物素标记核酸探针斑点杂交检测HBVDNA的敏感性、特异性和稳定性，用两种探针和(32)P标记探针进行平行检测对照．结果表明，地高辛探针检测灵敏度为0．1Pg，已达到(32)P中标记探针水平。生物素探针为1～10Pg。两种探针与32P标记探针相比。符合率分别96．65％和89．13％；敏感性分别为94．44％和83．33％；特异性分别为96．43％和92．86％。两种探针在－20℃存放6月后检测结果前后一致。研究结果表明，地高辛标记探针和生物素标记探针斑点杂交检测HBV．DNA都具有较高的敏感性、特异性和稳定性，其中以地高辛标记探针更优。To investigate the sensitivity, specificity and stability of the Dig-labelled and Biotin-labelled nucleiotide probes in the dot hybridization detection of HBV DNA. thses two probes were used to detect HBV DNA separately, and a 32 P-labelled probe was used as the control. The sensitivity of the Dig-labelled probe was 0. 1Pg, which was as high as that of the32 P-labelled, labelld was 1-10Pg. Compared with the 32 P-labelled probe, the positive coincidence cates of the Dig-labelled probe and the Biotin-labelled probe were 96. 65% and 89.13%, respectively; the sensitivities of them were 94. 44% and 83. 33%, respectively;and the specificities of them were 96.43% and 92. 86%, respetiveely. The detection results of thses two probes before before and after their storage at-20℃ for -6 months. had on difference. Both the Dig-labelled and Biotinlabelled probes had high sensitivities,specifitciyies and stabilities in detecting HBV DNA, but the Dig-labelled probe was better. biotin-labelled probe.

关 键 词：乙型肝炎病毒 DNA 地高辛标记探针 生物素标记 

分 类 号：R512.620.4[医药卫生—内科学]