白色假丝酵母菌基因核型和药敏表型的相关性  

Correlation between karyotype and fluconazole susceptibility of Candida Albicans

作　　者：雷红[1] 匡铁吉[1] 董梅[1] 佟爱华[1] 孟祥红[1] 何菊芳[1] 朱蕾[1] 王琰[1] 

机构地区：[1]解放军总医院第309临床部检验科,北京100091

出　　处：《军医进修学院学报》2008年第4期292-293,共2页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的:探讨临床白色假丝酵母菌基因核型和药敏表型的相关性。方法:应用脉冲场凝胶电泳(pulsed-field gel electrophoresis,PFGE)技术分析14株白色假丝酵母菌DNA染色体核型;采用ATB FUNGUS2 INT检测白色假丝酵母菌最低抑菌浓度(MIC);菌种鉴定采用MicroScanWalkAway96 RY-ID快速生化鉴定板。结果:从11例患者的不同标本、不同时期分离出14株白色假丝酵母菌进行基因核型分析,共具有9种不同核型。14株白色假丝酵母菌中有2株对氟康唑耐药(MIC>128μg.mL-1),其他12株对氟康唑均敏感(MIC<8μg.mL-1)。结论:在本研究中,基因核型相同或不同的白色假丝酵母菌菌株对氟康唑敏感性不同,表明对氟康唑耐药菌株与敏感菌株基因核型没有显著差异。Objective： To analyze the correlation between karyotype and fluconazole susceptibility of Candida albicans. Methods ： 14 isolates of C. albicans were evaluated for molecular relatedness by pulsed-field gel electrophoresis of chromosome- size DNA molecules. Minimal inhibitory concentration （MIC） of fluconazole against C. albicans was determined by ATB FUN- GUS2 INT; Isolates were identified by MicroScanWalkAway96 RY-ID. Results： Karyotype revealed a total of 9 different geno- types among 14 isolates of C. albicans from 11 patients. Among the 14 isolates, only two isolates were resistant to fluconazole （ 〉 128 μg·mL-1 ）. The rest 12 isolates were all sensitive to fluconazole （ 〈8 μg·mL-1）. Conclusion： In our study, there is no distinct difference of karyotype between fluconazole-resistant isolates and fluconazole-sensitive isolates.

关 键 词：白色假丝酵母菌 脉冲场凝胶电泳 氟康唑 药物敏感性试验 

分 类 号：R446.59[医药卫生—诊断学]