改良法制备异种脱蛋白骨复合重组人骨形态发生蛋白支架材料修复长骨大段骨缺损  

Repairing big segmental long bone defects in goat tibia using modified heterogeneous deproteinizated bone combined with recombinant human bone morphogenetic protein

作　　者：简月奎[1] 田晓滨[1] 赵筑川[1] 李波张美心，杨震 李波[1] 张美心[1] 杨震[1] 

机构地区：[1]贵州省人民医院骨科,贵州省贵阳市550002

出　　处：《中国组织工程研究与临床康复》2008年第27期5219-5222,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：贵州省科学技术基金(20063053)~~

摘　　要：背景：异种脱蛋白骨因其来源广泛和独特的生物学特性作为组织工程骨支架材料而受到较多关注。目的：采用改良法制备异种脱蛋白骨复合重组人骨形态发生蛋白支架材料,评价其修复大动物大段长骨缺损的成骨能力。设计、时间及地点：随机对照动物实验,于2005-05/2006-12在解放军第三军医大学实验动物中心及西南医院中心实验室完成。材料：市售猪股骨;山羊24只,10~12个月龄,雌雄不限。半环槽外固定器由解放军第三军医大学李起鸿教授研制,专利号：CN01256565。方法：山羊24只,随机分为单纯异种脱蛋白骨组、自体骨组及异种脱蛋白骨＋重组人骨形态发生蛋白组,在每只山羊右侧胫骨中下段截除胫骨总长度20%形成节段性骨缺损,按分组情况植入不同材料,采用半环槽式外固定器固定。异种脱蛋白骨制备参照文献完成。主要观察指标：术后4~24周每隔4周进行X射线及术后24周取新生骨进行双能X射线、组织学、生物力学检测骨缺损修复效果。结果：①制得的异种脱蛋白骨为三维多孔结构,其孔隙率为（78.5±6.45）%,孔径大小为（472.5±7.02）μm。②各组骨缺损表现为时间依从性的骨修复。生物力学试验抗压缩、三点抗弯曲、抗扭转试验,以及同一时间点平均成骨量均为自体骨组〉异种脱蛋白骨＋重组人骨形态发生蛋白组〉单纯异种脱蛋白骨组。③自体骨组、异种脱蛋白骨＋重组人骨形态发生蛋白组与正常组比较,山羊胫骨骨密度及骨矿含量、抗压缩压强及极限压强、抗弯曲载荷及极限载荷、抗扭转扭矩及极限扭矩平均值差异均无显著性意义。结论：改良法制备的异种脱蛋白骨复合重组人骨形态发生蛋白修复山羊胫骨大段缺损成骨能力与自体骨相当,可以作为组织工程支架材料。BACKGROUND： For its extensive sources and specific biological characteristics, deproteinized bone （DPB） gets more attention as the scaffold materials of bone tissue engineering. OBJECTIVE： To evaluate the ability of heterogeneous DPB prepared by modified method compound recombinant human bone morphogenetic protein （rhBMP2） to repair big segmental long bone defect of the animals. DESIGN, TIME AND SETTING： Randomized control animal experiment was completed in the Animal Center of the Third Military Medical University of Chinese PLA and the Central Laboratory of Southwest Hospital from May 2005 to December 2006. MATERIALS： Pig femor were used in this study, as well as 24 goats of 10-12 months old and either gender; Half-ring sulcated external fixator （Professor Li Qi-hong of the Third Military Medical University of Chinese PLA, Patent No： CN01256565）. METHODS： According to the implanted materials, 24 goats were randomly divided into 3 groups （n=6）, namely DPB group, autograft bone group and DPB＋rhBMP2 group. 20% segmental defects of right tibia middle and inferior bone were induced in every goat and then implanted with different materials. Half-ring sulcated external fixator was used for the fixation. DPB was prepared according to the references. MAIN OUTCOME MEASURES： Bone defect repairs were detected by X-ray photographs every 4 weeks during postoperative 4-24 weeks, and new bone executed dual-energy X-ray absorptiometry, histology and biomechanics at 24 postoperative weeks. RESULTS： The prepared DPB exhibited three-dimensional porous structure. The porosity was （78.5±6.45）%, and the pore size was （472.5±7.02） μ m. The bone repair in each group showed a time-dependent manner. The results of biomechanical test, such as torsional, bending and axial tensile tests, as well as the average new bone quantity in the autograft bone group was greater than that in the DPB＋rhBMP2 group, and that in the DPB group was the least. There was no significant difference of the goat tibia

关 键 词：异种脱蛋白骨 骨缺损 组织工程 支架材料 

分 类 号：R318.08[医药卫生—生物医学工程]