丝素蛋白支架材料复合骨髓间充质干细胞构建组织工程化软骨  

Constructing tissue engineered cartilage with silk fibroin compound bone marrow mesenchymal stem cells

作　　者：苗宗宁[1] 潘宇红[1] 祝建中[1] 钱寒光[1] 赵基栋[1] 

机构地区：[1]无锡市第三人民医院细胞实验室,江苏省无锡市214041

出　　处：《中国组织工程研究与临床康复》2008年第27期5243-5247,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景：一些研究已证明丝素蛋白是细胞立体培养的良好支架。目的：拟观察丝素蛋白作为支架材料复合骨髓间充质干细胞修复关节软骨缺损的可行性。设计、时间及地点：随机对照动物实验,于2006-08/2007-11在江苏省血吸寄生虫病防治研究所完成。材料：新西兰大白兔27只,雌雄不拘,体质量1.5~2.0kg,制备关节全层软骨缺损模型;丝素蛋白材料由苏州大学材料学院李明忠教授提供。方法：分离培养并定向诱导兔骨髓间充质干细胞分化为软骨细胞,与丝素蛋白膜材料复合培养,建立兔膝关节股骨髁间软骨缺损。27只大白兔随机抽签法分为3组,每组9只。复合组植入细胞/丝素蛋白材料复合物;丝素蛋白组植入单纯丝素蛋白材料,空白组不行任何植入。主要观察指标：扫描电镜观察细胞在材料上的生长情况;4,8,12周时取材观察软骨缺损修复情况。结果：骨髓间充质干细胞诱导后在材料上生长良好。骨髓间充质干细胞与丝素蛋白复合8周后,在兔膝关节内即形成软骨样细胞,且细胞外基质非常丰富,12周后与周围软骨色泽相近,表面光整,支架材料基本吸收,未见明显退变和淋巴细胞或白细胞浸润,所有标本均未见丝素蛋白残留。丝素蛋白组呈纤维软骨样修复,空白组基本没有修复。结论：用丝素蛋白复合骨髓间充质干细胞可形成透明软骨修复动物膝关节全层软骨缺损,显示了丝素蛋白材料作为关节软骨组织工程支架材料的良好生物相容性。BACKGROUND： Silk fibroin has been proved to be a good scaffold of three-dimensional culture of the cells. OBJECTIVE： To explore the feasibility of repairing the articular cartilage defects using silk fibroin with bone marrow mesenchymal stem cells. DESIGN, TIME AND SETTING： This study was a randomized control animal experiment and completed in Jiangsu Blood Fluke and Parasitosis Prevention and Cure Institute from August 2006 to November 2007. MATERIALS： Full-thickness defects in the femoral condyles were established in 27 New Zealand adult rabbits, which weighing 1.5-2.0 kg and irrespective of the genders. Silk fibroin was provided by Professor Li Ming-zhong from Materials College of Soochow University. METHODS： Rabbit bone marrow mesenchymal stem cells were isolated, cultured and induced to differentiate into chondrocytes, which were then cultured with silk fibroin membrane to create the defects in the femoral condyles of rabbit knee joints. Twenty-seven rabbits were divided into three groups at randomly, and every group contain nine rabbits. The compound group was implanted with cell/silk fibroin compound; Silk fibroin group was only implanted with silk fibroin; Blank group was left untreated. MAIN OUTCOME MEASURES： The growth condition of chondrocytes was observed by scanning electron microscope. The animals were sacrificed in 4, 8 and 12 weeks after operation, respectively to observe the reparation condition. RESULTS： The induced bone marrow mesenchymal stem cells grew well on silk fibroin membrane. In the compound group, chondrocyte-llke cells emerged in rabbit knee joints and extracellular matrix was extremely rich in 8 weeks; The color of repaired tissues was closed to surrounding cartilage. There was no evidence of the residue of silk fibroin and the infiltration of lymphocytes or leukocytes in 12 weeks specimens, and no obvious deterioration as well. In the silk fibroin group, defects were repaired with cartilago fibrosa and the defects were no repaired in the blank group. CONCLUSION： The

关 键 词：丝素蛋白 骨髓间充质干细胞 软骨 组织工程 

分 类 号：R318[医药卫生—生物医学工程]