中药部位复方DGH对STZ诱发的糖尿病大鼠早期视网膜形态学影响的实验研究  

Effect of DGH on retinal morphology impairment of STZ DM rats

作　　者：黄贵芝[1] 段俊国[2] 杨灵萍[1] 宋剑涛[3] 

机构地区：[1]浙江省杭州市第三人民医院眼科,杭州310009 [2]成都中医药大学眼科 [3]中国中医科学院眼科医院

出　　处：《中国中医眼科杂志》2008年第4期204-206,F0002,共4页China Journal of Chinese Ophthalmology

基　　金："863"计划重大项目(2002AA2Z3204)

摘　　要：目的观察中药部位复方DGH对链脲佐菌素(STZ)诱发的糖尿病大鼠早期视网膜损害的形态学影响,探讨中医药治疗糖尿病视网膜病变的病理机制。方法采用STZ诱发的糖尿病大鼠模型,分为5组:空白对照组、递法明组和DGH低、中、高剂量组,另设有正常对照组。连续灌胃3个月处死大鼠,其中每只大鼠左眼做视网膜组织切片、右眼视网膜消化铺片。结果DGH各组血糖水平有不同程度的下降。空白对照组毛细血管基底膜边界模糊不清,可见毛细血管壁断裂、多处局灶性多形核白细胞阻塞管腔,偶见闭锁的毛细血管等;DGH高、中、低剂量组、递法明组对基底膜内皮细胞增殖,基底膜增厚等有不同程度的改善,其中以高剂量组显著。DGH低剂量组、递法明组大鼠视网膜毛细血管周细胞明显凋亡,可见毛细血管局部扩张、膨隆,微血管瘤形成及渗透性改变,DGH高、中剂量组毛细血管周细胞数目减少,但毛细血管管腔基本正常,血管走向平直。结论DGH能抑制视网膜周细胞丧失及基底膜增厚,对DM大鼠视网膜微血管形态学损害有一定的保护作用,并在一定程度上能调控STZ诱发的糖尿病大鼠的血糖水平。OBJECTIVE To observe the effect of DGH on the early retinal morphology impairment of the experimental STZ DM rats,and to explore the pathology mechanism of how the Chinese medicine treating DR.METHODS Diabetic rats induced by streptozotocin（STZ） were randomly divided into 5 groups which were high,middle and low dosage of DGH,positive control group of Difral and normal group.Afterbeing given drugs or distilled water for 3 months,the rats were put to death;eyeballs of the rats were taken out.The left eye cup was proceeded tissue section,and the right eye cup was stretched preparation.RESULTS Both DGH′s high,middle dosage and Difral could decrease the thickness of RCBM of STZ DM rats;DGH′s high,middle dosage could decrease the pericyte loss of STZ DM rat′s;DGH could decrease the blood glucose of STZ DM rat′s in some degree.CONCLUSIONS DGH could inhibit pericyte apoptosis and decrease the thickness of RCBM.It was a good capillary protective agent;DGH could modulate the hyperglycemia situation of STZ DM rats.

关 键 词：中药部位复方DGH 链脲佐菌素 糖尿病性视网膜病变 视网膜形态学 

分 类 号：R285.5[医药卫生—中药学]