生物催化生成对苯二甲酸微生物协同作用的代谢途径分析  

Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis

作　　者：桑萍[1] 田晶[1] 高鹏[2] 许国旺[2] 

机构地区：[1]大连工业大学,大连116034 [2]中国科学院大连化学物理研究所国家色谱研究分析中心,大连116023

出　　处：《分析化学》2008年第8期1024-1028,共5页Chinese Journal of Analytical Chemistry

基　　金：国家973计划基金(Nos.2003CB716003,2004CB719605)资助项目;国家自然科学基金(No.20776029)资助项目

摘　　要：建立了对苯二甲酸、对甲基苯甲醛、对甲基苯甲醇、对甲基苯甲酸的高效液相色谱分析方法。采用HypersilSAX阴离子交换柱,流动相为2.5mol/LNH4H2PO4(含10%乙腈),pH4.32,流速0.8mL/min,柱温30℃,紫外检测波长为254nm。在此色谱条件下,各组分在7min内得到很好地分离,回收率符合测定要求。运用本方法测定了睾丸酮丛毛单胞菌和嗜麦芽窄食单胞菌生物催化生成对苯二甲酸不同发酵时间发酵液中主要代谢物含量。同时,采用GC-MS方法检测了有机酸、氨基酸、糖及长链脂肪酸等胞内代谢物,结合HPLC和GC-MS检测结果,分析了嗜麦芽窄食单胞菌和睾丸酮丛毛单胞菌协同作用催化对二甲苯生成对苯二甲酸的代谢途径。A method was developed for the determination of terephthalic acid, p-tolualdehyde, p-methylbenzyl alcohol and p-toluic acid by HPLC. Hypersil SAX column was used. The mobile phase was 2. 5 mol/L NH4H2PO4 containing 10 % acetonitrile, pH was 4.32. The flow rate was 0.8 mL/min, the temperature of column was 30 ℃ and detected wavelength was 254 nm. In this study, each component was completely separated and determined in 7 min. The recovery accorded with detecting requirement. The method was used to analyze the main metabolites in fermentation broth at different ferment times, which were produced at the process of biocatalysis producing terephthalic acid by Stenotrophomonas maltophilia and Comamonas testosteroni. The metabolites in cell including organic acids, amino acids, fatty acids and sugars were detected by GC-MS. According to the analytical results of HPLC and GC-MS, the pathway of producing terephthalic acid by stenotrophomonas maltophilia and comamonas testosteroni coordinated catalysis was discussed.

关 键 词：对苯二甲酸 高效液相色谱 生物催化 睾丸酮丛毛单胞菌 嗜麦芽窄食单胞菌 

分 类 号：TQ245.12[化学工程—有机化工] TQ033