On-line concentration of trace genistein by acid barrage stacking in capillary electrophoresis with UV detection  

作　　者：Hai Yan Feng Xiang Jun Li Shu Rong Hou Na Zheng Zhong Bo Hu Zhuo Bin Yuan 

机构地区：[1]College of Chemistry and Chemical Engineering, the Graduate University of the Chinese Academy of Sciences, Beijing 100049, China [2]School of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, China

出　　处：《Chinese Chemical Letters》2008年第8期973-976,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(No.20235010,20775088);the Foundation of State Key Laboratory of Electroanalytical Chemistry(No.2006002);the President Foundation of GUCAS.

摘　　要：A capillary electrophoresis method with UV detection was developed for high sensitively determining genistein. In this method, the online acid barrage stacking was applied. Four key factors influencing the stacking efficiency were systematically optimized. Genistein can be detected within 5 min at the concentration of 10 nmol/L, which was 300 times lower than that from conventional hydrodynamic injection. The repeatability, linear range, and limit of detection of the method were investigated with satisfactory result.A capillary electrophoresis method with UV detection was developed for high sensitively determining genistein. In this method, the online acid barrage stacking was applied. Four key factors influencing the stacking efficiency were systematically optimized. Genistein can be detected within 5 min at the concentration of 10 nmol/L, which was 300 times lower than that from conventional hydrodynamic injection. The repeatability, linear range, and limit of detection of the method were investigated with satisfactory result.

关 键 词：Acid barrage stacking Genistvin Online concentration Capillary electrophoresis Acidic compound 

分 类 号：Q13[生物学—普通生物学]