Simultaneous kinetic spectrophotometric determination of cephalexin and trimethoprim in pharmaceutical preparation and human urine with the aid of chemometrics  

作　　者：Yong Nian Ni Wei Qiang Xiao 

机构地区：[1]The Stake Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China [2]Department of Chemistry, Nanchang University, Nanchang 330047, China

出　　处：《Chinese Chemical Letters》2008年第8期981-984,共4页中国化学快报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China(No.20562009);the Natural Science Foundation of Jiangxi Province(No.0620041);the State Key Laboratory of the Chemo/Biosensing and Chemometrics of Hunan University(No.2005-22);the program for Changjiang Scholars and Innovative Research Team in Universities(No.IRT0540).

摘　　要：A procedure for the simultaneous kinetic spectrophotometric determination of cephalexin and trimethoprim was described. It was based on the different reaction rate of oxidation of these compounds with yellow ammonium cerous （Ⅳ） sulfate in acidic medium and colorless cerous （Ⅲ） sulfate was produced. The overlapped kinetic data was quantitatively resolved by the use of chemometric methods, partial least squares （PLS）, principal component regression （PCR） and radial basis function-artificial neural network （RBF-ANN）. The proposed method was also applied to the simultaneous determination of cephalexin and trimethoprim in pharmaceutical preparation and human urine with satisfied results, which compared well with those obtained by HPLC.A procedure for the simultaneous kinetic spectrophotometric determination of cephalexin and trimethoprim was described. It was based on the different reaction rate of oxidation of these compounds with yellow ammonium cerous （Ⅳ） sulfate in acidic medium and colorless cerous （Ⅲ） sulfate was produced. The overlapped kinetic data was quantitatively resolved by the use of chemometric methods, partial least squares （PLS）, principal component regression （PCR） and radial basis function-artificial neural network （RBF-ANN）. The proposed method was also applied to the simultaneous determination of cephalexin and trimethoprim in pharmaceutical preparation and human urine with satisfied results, which compared well with those obtained by HPLC.

关 键 词：Kinetic determination SPECTROPHOTOMETRY CHEMOMETRICS CEPHALEXIN TRIMETHOPRIM 

分 类 号：R97[医药卫生—药品]