吉西他滨纳米载药囊泡控释行为的研究  

Investigation into the controlled release behavior of gemcitabine-loaded nanovesicles

作　　者：郑建军[1] 贾林[1] 李皓[2] 黄开红[3] 帅心涛[2] 李瑜元[1] 

机构地区：[1]广州医学院附属广州市第一人民医院消化内科,510180 [2]中山大学化学与化学工程学院高分子研究所 [3]中山大学附属第二医院消化内科

出　　处：《中华医学杂志》2008年第28期2005-2007,共3页National Medical Journal of China

基　　金：广东省社会发展领域科技计划基金资助项目（2006-63082）

摘　　要：目的制备吉西他滨（Gemcitabine）纳米载药囊泡，对其表面形态、粒径分布、微粒结构、体外释放等性能进行评估。方法以两亲性共聚物聚乙二醇-聚乳酸（PEG—PDLIA）为原料，采用双乳化法制备载药囊泡，Gemcitabine为模型药物。通过电子显微镜观察纳米载药囊泡的形态和结构，用紫外可见分光光度计测定囊泡的载药量、包封率和体外释放量。结果纳米载药囊泡呈球形或近似球形，具有明显的空心结构，平均粒径为200．6nm，载药量和包封率分别为4．14％和20．54％，体外释放实验表明该囊泡具有良好的控释特性。结论以PEG—PDLLA为原料制备的Gemcitabine纳米载药囊泡具有良好的控释性能，为胰腺癌动物体内实验研究提供了可靠的依据。Objective To prepare gemcitabine-loaded nanovesicles and to observe its morphology, structure, particle size, and drug-release performance in vitro. Methods Diemulsion technique was used to prepare nanovesicles as carrier from amphiphilic block copolymer of poly （ethylene glycol）-block-poly （D, L-lactide） , and gemcitabine was used as the model drug. The morphology of vesicles was determined by scanning electron microscope （SEM） and transmission electron microscope （TEM） , and its drug loading （DL）, encapsulation ratio （ER）, and drug-release curve in vitro were detected by UV-Vis-NIR Spectrophotometer. Results The Gemcitabine-loaded nanovesicles is a kind of hollow nanosphere with the mean size of 200.6 nm, DL of 4. 14% and ER of 20. 54%. The nanovesicles showed its excellent controlled-release characteristic in the experiment of drug release in vitro. Conclusion The nanovesicles prepared from PEG-PDLLA can be served as one of carriers for Gemcitabine with good performance of drug controlled-release. It will provide a reliable experimental base for the further researches in vivo.

关 键 词：纳米囊 给约系统 GEMCITABINE 

分 类 号：R686[医药卫生—骨科学]