APA微囊膜通透性的研究  

THE PERMEABILITY OF APA MICROENCAPSULE MEMBRANE

作　　者：林常敏[1] 李宇[1] 黄铿[1] 纪影畅[1] 蔡湘娜[1] 李国强[1] 

机构地区：[1]汕头大学医学院第一附属医院,广东汕头515041

出　　处：《现代医院》2008年第9期16-17,共2页Modern Hospitals

摘　　要：目的激光扫描共聚焦显微镜测定APA(alginate-polylysine-alginate,海藻酸钠-多聚赖氨酸-海藻酸钠)微囊膜的通透性。方法采用荧光素标记的分子量分别为10 ku、40 ku、70 ku的葡聚糖与APA微囊共同孵育,观察形成的激光共聚焦图像,检测相同时间、不同直径的APA微囊中葡聚糖-荧光素的强度。结果荧光素在APA微囊中呈同心圆状、逐层渗透的方式扩散,在相同时间、同一层面观察,分子量越大,通透的速度越慢,相同分子量、相同时间内荧光强度比较:小囊组>中囊组>大囊组(p<0.05)。结论APA微囊既可允许小分子的物质通过,保证营养物质、代谢产物和信号分子的自由进出,又能截割大分子物质,起到免疫隔离作用。Objeetive To determine the permeability of APA microcapsule by using laser scanning cortfocal mierseope. Methods Three molecular weight ：10ku,40ku, 70ku were selected in this study. Their diffusion way and speed into the mieroeapsulations were observed by eonfoeal laser scanning microscopy. The comparison of fluo- renseein intensity inside the mieroeapsulations was made among difference diameters of APA microeapsulations. Re- suits The fluorenscein was diffused gradually into the mieroeapsulations with a shape of concentric circularity： The fluorenseein intensity inside three groups of APA mieroeneapsulations were： small 〉 middle 〉 big （p 〈 0. 05）. Condusions The APA mleroeneapsulation ensures the nutrition and metabolite to pass in and out freely, and isolats the immunoeompetent substance absolutely.

关 键 词：APA微囊 通透性 毛乳头 

分 类 号：R392[医药卫生—免疫学] R135.11[医药卫生—基础医学]