应用基因芯片技术研究日本曲霉(Aspergillus japonicus)化感物质SAF抑制植物生长的分子机理  

The research of molecular mechanism on SAF from Aspergillus japonicus in inhibiting plant growth using gene chip technique

作　　者：李明[1] 曾任森[1] 骆世明[1] 

机构地区：[1]华南农业大学热带亚热带生态研究所

出　　处：《生态学报》2008年第8期3891-3897,共7页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(30570283);中国博士后科学基金资助项目(4100-C05014);广东省自然科学基金资助项目(05300830);华南农业大学校长基金资助项目(2005K029)~~

摘　　要：利用基因芯片技术研究日本曲霉的化感物质SAF化感作用的分子机理,结果表明,SAF具有浓度效应,能诱导受体植物-拟南芥的多种调控细胞死亡相关基因的上调表达,高浓度处理表达的基因数量和倍数均高于低浓度处理的。此外,SAF也能诱导与防御反应相关基因的上调表达,高浓度处理表达的基因种类和倍数均低于低浓度处理的。SAF能诱导生长素响应基因、编码乙烯反应敏感蛋白等基因的上调表达。由实验结果推测,SAF具有生长素的性质,高浓度处理可以导致受体细胞中乙烯的积累,并引起对维持细胞正常稳态起重要作用的物质-神经酰胺水平的改变,从而产生受体植物叶片的衰老和死亡的结果,这是SAF具有化感作用的主要原因。研究对于揭示SAF化感作用的分子机理具有重要的意义,对于阐明寄主非选择性毒素作用的分子机理也提供参考依据。The investigation was conducted to detect the inhibition effects of secalonic acid F （SAF） produced by Aspergillus japonicus on higher plant by using gene chip technique, the effects of SAF depended on its concentration, many genes associated with cell death were up-regulated after treated by SAF, the number and expression levels induced by higher SAF concentration were higher than those induced by lower concentration. Genes associated with defense function were also up-regulated by SAF, however, gene number and expression levels induced by higher SAF concentration were lower than those treated by lower concentration. In addition, results showed that SAF acted as auxin, high concentration of SAF might induce the production of ethylene and affact ceramide content which might result in senescence and death in the leaves of receptor plant. The findings brought some new clues for further studying the action mechanism of SAF and non-host selection toxin.

关 键 词：SAF 基因芯片 细胞死亡 化感作用 

分 类 号：Q943[生物学—植物学]