补肾阳中药对小鼠胚胎干细胞活动及相关基因表达的影响  

Effects of nourishing kidney-yang on the activities of embryonic stem cell and related gene expression

作　　者：安红梅[1] 胡兵[1] 史云峰[1] 张占鹏[1] 许丽雯[1] 顾耘[1] 潘露茜[1] 王志[2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学中药学院,上海201203

出　　处：《中华中医药杂志》2008年第9期774-776,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30400574)

摘　　要：目的:观察补肾阳中药复方对小鼠胚胎干细胞活动的影响。方法:选用小鼠胚胎干细胞模型CRL-1825细胞株,加入补肾阳中药复方大鼠含药血清,观察补肾阳中药对干细胞增殖、分化、凋亡以及相关基因表达的影响。结果:补肾阳中药可以抑制CRL-1825细胞凋亡,但对CRL-1825细胞增殖、分化无明显影响;补肾阳中药同时可促进CRL-1825细胞Notch1基因表达,相对表强度由46.33升高至102.81;补肾阳中药还可以抑制P16INK4a基因表达,相对表强度由202.11降至93.11。结论:补肾阳中药复方可以抑制CRL-1825细胞凋亡,并可能与调控Notch1、P16INK4a表达相关。Objective： To observe effects of nourishing kidney-yang on activities of embryonic stem cell. Method： Embryonic stem cell line CRL-1825 was used as a model. Serum of kidney-yang-nourishing herbals was added to CRL-1825 medium, and the effects of kidney-yang-nourishing herbals on CRL-1825 cell proliferation, differentiation, apoptosis and related gene expression were observed. Result： Kidney-yang-nourishing herbals could inhibit the CRL-1825 cell apoptosis, without influences on CRL-1825 cell proliferation and differentiation; it also up-regulates Notchl expression and down-regulates PI6INK4a expression. Conclusion： Nourishing kidney-yang could inhibit CRL-1825 cell apoptosis, it may relate to Notchl and P16INK4a expression.

关 键 词：补肾阳中药 胚胎干细胞 凋亡 NOTCH P16^INK4A 

分 类 号：R285.5[医药卫生—中药学] R49[医药卫生—中医学]