大黄虫丸抗血小板活化的机制研究  

Mechanism study on Dahuangzhechong pill antiplatelet activation

作　　者：王东生[1] 陈方平[2] 贺石林[3] 肖长江[2] 唐发清[2] 

机构地区：[1]中南大学湘雅医院中南大学中药筛选工程技术中心中南大学材料科学与工程博士后站,长沙410008 [2]中南大学湘雅医院,长沙410008 [3]中南大学湘雅医学院,长沙410008

出　　处：《中华中医药杂志》2008年第9期818-821,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中国博士后科学基金(No.20060390891;No.2003033474);湖南省中医管理局资助项目(No.6302-23);湖南省科技厅资助项目(No.2006FJ4243)

摘　　要：目的:探讨大黄虫丸抗血小板活化的作用。方法:用大黄虫丸含药血浆孵育血小板,然后用ADP诱导活化,血小板膜糖蛋白GPⅡb/Ⅲa复合物纤维蛋白原受体(PAC-1)单克隆抗体标记,流式细胞术分析血小板的活化。同时,冠心病及脑梗死患者服用大黄虫丸,治疗1疗程(30d)后,取患者血小板,采用流式细胞术分析患者血小板的活化。结果:大黄虫丸药物体外血浆组血小板活化率为20.82%,阿司匹林组血小板活化率为51.71%,大黄虫丸组血小板活化明显低于阿司匹林组(P<0.01)。在临床治疗冠心病试验中,服用大黄虫丸患者血小板活化率为14.78%,明显低于服用阿司匹林患者血小板活化率67.74%(P<0.01);对于脑梗塞患者,服用大黄虫丸患者血小板活化率为5.65%,低于阿司匹林患者组77.91%(P<0.01)。结论:大黄虫丸能较好的抑制血小板活化,可能是理想的抗动脉血栓形成药物。Objective： To investigate the inhibitory effect of Dahuangzhechong pill to platelet activation. Methods： The blood platelet were incubated with the medicated blood plasma with Dahuangzhechong pill, and then activated with ADP and marked with PAC- 1 monoclonal antibody. The activation rate of blood platelet was analyzed by flow cytometer. The patients with coronary heart disease or cerebral infarction took Dahuangzhechong pill, after one course of treatment, the patients, blood platelet were separated and then incubated with PAC- 1 monoclonal antibody. The activation of blood platelet was detected by flow cytometer. Results： Compared with aspirin group（51.7%）, the activation rate （20.82%） of blood platelet in Dahuangzhechong pill group decreased （P 〈 0.01）. In the clinic trail of coronary heart disease, the blood platelet activation rate （14.78%） in Dahuangzhechong pill group was lower than that in aspirin group （67.74%） （ P 〈 0.01 ） . For cerebral infarction, compared with aspirin group （77.91%）, the platelet activation rate in Dahuangzhechong pill group decreased （5.65%） （P 〈 0.01 ） .Conclusion： Dahuangzhechong pill has a satisfactory effect in anti-platelet activating.

关 键 词：大黄[庶虫]虫丸 血小板活化 动脉血栓形成 

分 类 号：R285.5[医药卫生—中药学]