玻璃化保存法对肌腱形态学及生物力学影响  

Observation of Histomorphology and Biomechanics on Tendons Preserved by Vitrification

作　　者：王文良[1] 刘英杰[1] 张华亮[1] 陈光[1] 

机构地区：[1]武警医学院附属医院,天津300162

出　　处：《医疗卫生装备》2008年第8期6-8,共3页Chinese Medical Equipment Journal

基　　金：天津市应用基础研究计划面上项目(05YFJMJC13900)

摘　　要：目的:探讨玻璃化保存法对兔肌腱组织、细胞形态及力学性能的影响。方法:以18.64%二甲基亚砜(V/V)+13.37%乙酰胺(V/V)+9.17%1,2丙二醇(V/V)+0.10 mmol/L海藻糖+10%小牛血清为玻璃化冷冻保护剂,将新西兰纯种大白兔肌腱采用三步法预处理,-196℃液氮保存14 d作为玻璃化保存组;以15%二甲基亚砜+10%小牛血清作为冷冻保护剂,"两步法"处理后-196℃液氮冷冻保存14 d的新西兰纯种大白兔胫前肌肌腱作为对照组,透射电镜观察组间肌腱组织细胞的形态差异,并分别进行肌腱拉伸实验,检测肌腱破坏载荷峰值、最大载荷拉伸位移及杨氏弹性模量。结果:玻璃化保存组细胞的结构形态未见明显改变,对照组肌腱组织微观结构损伤明显。肌腱破坏荷载峰值:玻璃化保存组与对照组无统计学意义(P=0.256);最大载荷拉伸位移:玻璃化保存组与对照组间差异无统计学意义(P=0.065);杨氏弹性模量:玻璃化保存组与对照组间差异有统计学意义(P=0.006)。结论:与对照组肌腱相比,玻璃化保存法对肌腱组织细胞形态损伤较小,但两者在生物力学特性上无明显差异。Objective To investigate the effect of vitrification on the biomechanics of tendon tissue in rabbit. Methods Two frozen methods were adopted. The first group was treated with Cryoprotective Agent, which was composed of 18.64% DMSO（V/V）, 13.37% Acetamide （V/V）, 9.17% 1, 2 Propylene glyco （V/V）, 0.10mmol/L Trehalose and 10% Calf serum . The tendon tissue with three steps of preliminary treatment, preserved in Cryoprotective Agen was conserved in liquid nitrogen （-196℃）for 14 days; The second group treated with 15% DMSO and 10% Calf serum served as control group. Transmission electron microscope was used to observe the Histiooytic shape of tendon. Each group was performed with tendon tensile test, which could detect maximum load, the maximum shifting quantity and Young＇s Elastic Modulus. Results There was no significant damage in the tissue＇s micromechanism of vitrified tendon. But in cryopreservation group, the tissue＇s micromechanism was apparently damaged. There was no significant difference between test group and control group in maximum load （P=0.256）. The same was the maximum shifting quantity （P=0.065）. There was significant difference between test group and control group in Young＇s Elastic Modulus （P=0.006）. Conclusion The damage of vitrification to tendon is less than that of profound hypothermia preservion, especially to tendon＇s corpuscular shape, but there is no significant difference between test group and control group in tendon＇s biomechanics.

关 键 词：肌腱 形态学 生物力学 玻璃化 冷冻保存 

分 类 号：R318.52[医药卫生—生物医学工程]