HPLC-MS/MS快速检测动物尿液中的莱克多巴胺和克伦特罗  

Rapid Determination of Ractopamine and Clenbuterol in Animal Urine with HPLC-MS/MS

作　　者：聂建荣[1] 洪振涛[1] 连槿[1] 邓香连[1] 

机构地区：[1]广州市农业标准与监测中心,广州510315

出　　处：《中国兽药杂志》2008年第8期17-20,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了以高效液相色谱-质谱（HPLC—MS／MS）快速检测动物尿液中的莱克多巴胺和克伦特罗分析方法。样品经SCX柱净化浓缩后，以乙腈-5mmol／L乙酸铵（65：35，V／V）为流动相，采用Kromasil CN色谱柱分离，通过电喷雾（ESI）离子源电离多反应监测（MRM）对莱克多巴胺和克伦特罗作定性和定量分析。在1．0～500．0μg／L的范围内线性良好（r〉0．999）。在0．5、4．0、40．0、80．0、200．0μg／L添加水平下，回收率在89．7％～103．3％，相对标准偏差为2．0％～7．8％。检测限（S／N=5）为0．1μg／L。The HPLC- MS/MS method was established to analyze ractopamine and clenbuterol in animal urine. The sample was purified and concentrated by SCX column, chromatographic conditions included a Kromasil CN column with the mobile phase of acetone- 5 mmol/L ammonium acetate（65： 35, V/V）. Qualitative and quantitative analysis of the ractopamine and clenbuterol which ionized by electrospray ionization interface （ESI） was carried on through MRM mode. There was a good linear correlation in the range of 1.0 -500.0 μg/L（r 〉0. 999）. The recoveries ranged from 89.7% to 103.3% at the add concentration level of 0. 5 ,4. 0,40. 0,80. 0,200. 0 μg/L. The RSD was between 2.0% and 7.8%. Its limit of detection was 0.1 μg/L.

关 键 词：HPLC-MS/MS 动物尿液 莱克多巴胺 克伦特罗 

分 类 号：S859.84[农业科学—临床兽医学]