双参素胶囊对大鼠实验性心肌缺血再灌注损伤的保护作用  

Protective effects of Shuangshensu capsules on experimental myocardial ischemiareperfusion injury in rats

作　　者：刘兵[1] 杨春梅[1] 于晓风[2] 曲绍春[2] 徐华丽[2] 睢大员[2] 

机构地区：[1]武警吉林总队医院,长春130052 [2]吉林大学药学院药理教研室,长春130021

出　　处：《武警医学》2008年第8期713-716,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：吉林省科技厅新药基金项目资助课题(20000329)

摘　　要：目的观察双参素胶囊对大鼠实验性心肌缺血再灌注损伤的保护作用。方法通过在体大鼠结扎左冠状动脉前降支30 min,再灌注24 h制备实验性心肌缺血再灌注损伤模型,双参素胶囊按17.5 mg/kg3、5 mg/kg7、0 mg/kg给大鼠连续灌胃7 d,阳性药物组连续灌胃福辛普利20 mg/kg,7 d,假手术组及再灌模型组灌胃0.5%羧甲基纤维素钠,计算心肌梗死范围(MIS),测定血清天冬酸氨基转移酶(AST)等的活性。结果双参素胶囊可使实验性心肌缺血再灌注损伤大鼠的MIS明显缩小(P<0.05或P<0.01),血清AST、LDH、CK-MB活性及MDA含量明显降低(P<0.05或P<0.01),血清SOD及GSH-Px活性明显增加(P<0.05或P<0.01),亦可使血浆ET和AngⅡ含量明显下降(P<0.05或P<0.01)。结论双参素胶囊对大鼠实验性心肌缺血再灌注损伤具有明显保护作用。Objective To study the protective effects of Shuangshensu capsules （SSC） on experimental myocardial ischemia - repeffu- sion injury in rats.Methods The rat myocardial ischemiareperfusion model was developed by 30 min left anterior descending coronary occulusion and 24 h reperfusion in open chest anesthetized rats. The activities of aspartate aminotransferase （AST） and other Myocardial enzymes were determined. Results In rats treated with SSC （35 and 70 mg/kg i. g for 7 d）, the myocardial ischemia size was significantly reduced （ P 〈 0.05 or P 〈 0.01）, the AST, LDH and CK - MB activities and the MDA content in serum declined （ P 〈 0.05 or P 〈 0.01 ）, while the SOD and GSH- Px activities in serum increased significantly （ P 〈 0.05 or P 〈 0.01）. In addition,the ET and Magl] levels in plasma also declined markedly. Conclusion SSC have protective effects on myocardial ischemia - reperfusion injury.

关 键 词：双参素胶囊 心肌缺血再灌注损伤 心肌梗死范围 心肌酶 自由基 血管内皮细胞因子 

分 类 号：R285.5[医药卫生—中药学] R541.4[医药卫生—中医学]