大鼠原位肝移植模型的手术技巧  

Operation skills for Orthotopic Liver Transplantation in Rats

作　　者：王振猛[1] 唐乙[2] 宋少华[3] 陆智杰[1] 王全兴[2] 俞卫锋[1] 

机构地区：[1]上海东方肝胆外科医院麻醉科,上海200438 [2]解放军第二军医大学基础部免疫学教研室,上海200433 [3]上海长征医院移植科,上海200003

出　　处：《临床军医杂志》2008年第4期638-640,共3页Clinical Journal of Medical Officers

摘　　要：目的探讨建立稳定的大鼠原位肝移植模型的外科手术技巧,为研究肝脏缺血再灌注损伤提供技术支持。方法110只雄性SD大鼠随机配对后分别作为供体和受体,通过改良Kamada"二袖套"法施行大鼠原位肝移植。结果改良Kamada法建立大鼠原位肝移植模型共52例,7d生存率88.5%,7例长期存活大于100d。结论大鼠原位肝移植模型的建立需要极度的耐心和娴熟的手术技巧,模型的建立有助于研究肝脏缺血再灌注损伤,移植免疫及器官保存。Objective To improve the operation technique for orthotopic liver transplantation in rats, and illustrate the hepatic ischemia-reperfusion injury in future study.Methods One hundred and ten male SD rat were randomly paired as donor and receptor and were performed with orthotopic liver transplantation by modified Kamada＇s two cuffs technique.Results As a result, 88.5% of the 52 rats with orthotopic liver transplantation survived more than 7 days and 7 ones survived longer than 100 days. Conclusion Extreme patience and skillful surgical techniques are necessary for rat orthotopic liver transplantation model, which is helpful to the study on hepatic ischemia-reperfusion injury, transplantation immunity and organ preservation.

关 键 词：原位肝移植 动物模型 大鼠 

分 类 号：R617[医药卫生—外科学] R657.3[医药卫生—临床医学]