辽河油田冻融石油污染土壤中原位修复微生物  

In-situ remediation of microorganisms in frozen and thawed petroleum-contaminated soil from Liaohe Oil Field, Liaoning Province

作　　者：李素玉[1] 李法云[2] 张志琼[1] 罗岩[1] 杨姝倩[2] 魏伯峰[1] 

机构地区：[1]辽宁大学生命科学学院,辽宁沈阳110036 [2]辽宁大学环境学院,辽宁沈阳110036

出　　处：《辽宁工程技术大学学报（自然科学版）》2008年第4期599-601,共3页Journal of Liaoning Technical University (Natural Science)

基　　金：国家自然科学基金资助项目(30570342);国家重点基础研究发展计划资助项目(2004CB418506);国家教育部留学回国人员科研启动基金资助项目(教外司留[2005]383);辽宁省高校污染控制与环境修复重点实验室与辽宁省环境保护开放实验室联合开放基金资助项目

摘　　要：以辽河油田不同年代开采油井附近采取的土壤样品为研究对象,通过冻融模拟试验和采用选择性培养基筛选对石油污染土壤具有原位修复作用的优势微生物菌株,并比较油田冻结土壤与未冻结土壤中石油降解细菌与真菌的数量变化特征及其对石油的降解能力。结果表明,从未冻结土壤中筛选出石油降解细菌14株、石油降解真菌6株;从冻结土壤中筛选出石油降解细菌5株、石油降解真菌2株。石油降解细菌假单胞菌属(Pseudomonas sp.)的B3号菌株在37℃、7天时间内对石油的降解率最高,为30.2%;石油降解真菌木霉属(Trichoderma sp.)的F3号菌株在28℃、7天时间内对石油的降解率最高,为47.2%。This paper compares variability of petroleum biodegradation bacteria in frozen and thawed soil collected from Liaohe Oil Field and their capacities for biodegrading petroleum were also determined. The results show that 14 strains of bacteria and 6 strains of fungi for petroleum biodegradation were screened from thawed soils, while only 5 strains bacteria and 2 strains fungi existed in freeze soils. It presents that microorganism diversities in thawed soil is more than that in frozen soil, and that different species of microorganism response to low temperature distinctly. The experiments of petroleum biodegradation indicate that B3 bacterium （Pseudomonas sp.） has the capacity of 30.2 percent to biodegrade petroleum under the condition of 37 centigrade after 7 day＇s incubation. The petroleum biodegradation capacity for F3 fungus （Trichoderma sp. ） is 47.2 percent under the condition of 28 centigrade after 7 days incubation.

关 键 词：石油污染土壤 石油降解微生物 冻融 

分 类 号：X703[环境科学与工程—环境工程]