滋阴泻火中药对雌性青春期大鼠生长板ERα、IGF-1R及EGFR基因表达和蛋白合成的调节作用  

Regulative Effects of Chinese Herbs for Nourishing Yin and Removing Fire on Gene Expressions of Estrogen Receptor α,Insulin-like Growth Factor-1 Receptor,Epithelial Growth Factor Receptor and Protein Synthesis in Epiphyseal Growth Plate of Female Puberta

作　　者：芦军萍[1] 蔡德培[1] 

机构地区：[1]复旦大学附属儿科医院,上海200032

出　　处：《中国中西医结合杂志》2008年第8期721-724,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30371820)

摘　　要：目的观察滋阴泻火中药对雌性青春期大鼠生长板雌激素受体α(ERα)、胰岛素样生长因子-1受体(IGF-1R)及表皮细胞生长因子受体(EGFR)基因表达和蛋白合成的调节作用,探讨滋阴泻火中药改善骨骼发育的作用环节和作用机制。方法将青春期大鼠分为对照组和中药干预组,采用免疫组织化学和实时定量-聚合酶链反应(Realtime-PCR)对干骺端生长板ERα、IGF-1R和EGFR的基因表达和蛋白合成情况进行检测。结果给予滋阴泻火中药干预后,青春期大鼠干骺端生长板ERα和IGF-1R的基因表达明显下调,蛋白合成显著减少;EGFR的基因表达和蛋白合成无明显改变。结论滋阴泻火中药可以通过调节生长板ERα和IGF-1R的表达,对骨骼的发育和成熟发挥调节作用。Objective To investigate the effect of Chinese herbs for nourishing yin and removing fire （NYRF） on gene expressions of estrogen receptor α （ERos） , insulin-like growth factor-1 receptor （IGF-1R） and epithelial growth factor receptor （EGFR） in the epiphyseal growth plate of the female pubertal rats. Methods The rats were randomly divided into the control group and the intervened group. Immunohistochemistry and reahime- PCR methods were used to measure the gene expression of ERos, IGF-1 R and EGFR and their protein synthesis in epiphyseal growth plate. Results After being intervened with NYRF, the gene expressions of ERc~ and IGF-1R were down-regulated and their protein synthesis markedly reduced, while those of EGFR were unchanged. Conclusion NYRF can modulate the development and maturation of bone by regulating the expressions of ERα and IGF- 1 R in the epiphyseal growth plate.

关 键 词：滋阴降火中药 生长板 雌激素受体α 胰岛素样生长因子-1受体 表皮细胞生长因子受体 

分 类 号：R285.5[医药卫生—中药学]