PAAF与ASPAAF诱导的肠损伤比较的实验研究  

Comparison of The Injury of Intestines Induced by PAAF and ASPAAF in Rats

作　　者：刘震[1] 程若川[2] 方向[1] 张跃天[1] 赵海剑[2] 李俊[1] 陈玉祥[1] 

机构地区：[1]四川省德阳市人民医院普外科,德阳618000 [2]昆明医学院第一附属医院普外科,昆明650032

出　　处：《中国普外基础与临床杂志》2008年第8期555-559,共5页Chinese Journal of Bases and Clinics In General Surgery

基　　金：四川省卫生厅立项课题(编号:070286)~~

摘　　要：目的探讨胰腺炎相关性腹水(PAAF)与急性化脓性腹膜炎相关性腹水(ASPAAF)诱导大鼠肠损伤的作用及其可能机理。方法以PAAF和ASPAAF分别诱导的SD大鼠肠损伤模型为研究对象,将48只SD大鼠随机均分为3组。对照组腹腔内注射生理盐水8ml/只;PAAF组腹腔内注射PAAF8ml/只,ASPAAF组腹腔内注射ASPAAF8ml/只。分别于注射后6h和12h分批处死大鼠,每时间点8只。测定血清内毒素和TNF-α水平,同时测定小肠组织中Na+-K+-ATP酶活性及TNF-α水平;于光镜下观察肠黏膜损伤情况并作等级比较。结果注射后6h和12h,PAAF组和ASPAAF组血清内毒素和TNF-α水平,小肠组织中TNF-α水平以及肠黏膜损伤程度均较对照组增高(P<0.05),而Na+-K+-ATP酶活性则显著降低(P<0.05);ASPAAF组血清内毒素和TNF-α水平,小肠组织中TNF-α水平以及肠黏膜损伤程度又均较PAAF组增高(P<0.05),Na+-K+-ATP酶活性则显著降低(P<0.05)。结论PAAF及ASPAAF均能引起大鼠肠损伤,且ASPAAF引起的损伤更为严重。Objective To explore the role and intrinsic mechanism of the injury of intestines induceded by pancreatitis associated ascitic fluid （PAAF） and acute suppurative peritonitis associated ascitic fluid （ASPAAF） in rats. Methods Forty-eight Sprague-Dawley （SD） rats, male or female, were randomly divided into three groups averagely. The control group： 8 ml of normal saline （NS） was injected into the peritoneal cavity; the PAAF group： 8 ml of PAAF was injected into the peritoneal cavity; and the ASPAAF group： 8 ml of ASPAAF was injected into the peritoneal cavity. After peritoneal cavity injection, the rats were put to death in batches at 6 h and 12 h, eight rats per-batch. Levels of TNF-α and endotoxin in serum were measured. The activity of ATP enzyme and level of TNF-α in the intestinal tissues were measured. The pathological changes of intestines were observed by microscope. Results The revels of TNF-α, endotoxin and the degree of injury of the intestines were markedly elevated and the activity of ATP enzyme of the intestinal tissues was decreased in the PAAF group and ASPAAF group compared with those in the control group （P〈0.05）. The levels of TNF-α, endotoxin and the degree of injury of the intestines were markedly elevated and the activity of ATP enzyme of the intestinal tissues was decreased in the ASPAAF group compared with those in the PAAF group （P〈0.05）. Conclusion PAAF and ASPAAF can induce the injury of in-testines, but the injury of intestines induced by ASPAAF is more serious.

关 键 词：胰腺炎相关性腹水 急性化脓性腹膜炎相关性腹水 肠黏膜损伤 肿瘤坏死因子-Α 

分 类 号：R657.51[医药卫生—外科学]