粗山羊草抗条锈病新基因YrY206遗传分析和微卫星标记  被引量:8

Genetic Analysis and SSR Mapping on an New Stem Stripe Rust Resistance Gene YrY206 in Aegilops tauschii

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作  者:张海泉[1] 郎杰[1] 马淑琴[2] 张宝石[3] 

机构地区:[1]河北经贸大学生物科学与工程学院,石家庄050061 [2]云南省农科院粮作所,昆明650205 [3]沈阳农业大学农学院,沈阳110161

出  处:《生物工程学报》2008年第8期1475-1479,共5页Chinese Journal of Biotechnology

基  金:河北省科学技术研究与发展指导计划(No.05225510)资助~~

摘  要:从小麦野生近缘属——粗山羊草中挖掘小麦条锈病抗病基因,拓展小麦抗病性的遗传基础。利用抗小麦条锈病与感小麦条锈病的粗山羊草间杂交,从粗山羊草[Aegilops tauschii(Coss.)Schmal]Y206中鉴定出1个显性抗小麦条锈病基因,暂定名为YrY206。应用分离群体分组法(Bulked segregant analysis,BSA)筛选到Wmc11a、Xgwm71c、Xgwm161和Xgwm183标记,与该基因之间的遗传距离分别为4.0、3.3、1.5和9.3cM。根据连锁标记所在小麦微卫星图谱的位置,YrY206被定位在3DS染色体上。分析基因所在染色体的位置、抗病性特征,认为YrY206是一个新的抗小麦条锈病基因。A wheat stripe rust resistance gene was screened out from Aegilops tauschii which is relative genera of wheat species, broadening the genetic basis of the anti-disease character of wheat species. By hybridizing diversed Ae. Tauschii species, which is either resistant or susceptible to wheat stripe rust, a dominant wheat stripe rust resistance gene was detected from Ae. Tauschii (Coss.) Schmal Y206. The novel gene was temporarily designated as YrY206. By bulk segregation analysis, four microsatellite markers Wmc 11 a, Xgwm71 c, Xgwm 161 and Xgwm 183 were found to be linked to YrY206 with genetic distances of 4.0, 3.3, 1.5 and 9.3 cM, respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 3DS. Based on the chromosomal location and the resistance pattern of the gene, YrY206 should be a novel stripe rust resistance gene.

关 键 词:粗山羊草 小麦条锈病 基因定位 

分 类 号:S512.1[农业科学—作物学]

 

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