转人α-乳清白蛋白基因烟草中半胱氨酸含量的提高(英文)  

Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants

作　　者：王逸群[1] 

机构地区：[1]福建师范大学生命科学学院发育与神经生物学重点实验室,福建福州350108

出　　处：《云南植物研究》2008年第4期457-463,共7页Acta Botanica Yunnanica

基　　金：the scientific and technological program of Fujian Science and Technology Department(2004N026);the Natural Science Foundation of Fujian Province(B0410009);the scientific and technological program of undergraduate students in Fujian Normal university(BKL2007062)

摘　　要：将人α-乳清白蛋白基因构建到植物表达载体上,通过农杆菌介导采用叶盘法将人α-乳清白蛋白基因导入到烟草中,经过PCR和Southern blot分析表明:人α-乳清白蛋白基因已经整合到烟草基因组中;经过RT-PCR和GUS组织化学染色证明:人α-乳清白蛋白基因得到了表达。测定了9株转基因烟草叶片半胱氨酸含量,大部分植株有着明显的提高,最高幅度达到了318.02%,半胱氨酸含量平均提高了166.40%。The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%.

关 键 词：人α-乳清白蛋白基因 转基因烟草 半胱氨酸 提高 

分 类 号：Q943.2[生物学—植物学]