冷暴露对中缅树鼩褐色脂肪组织中解偶联蛋白1含量的影响  

The Effects of Cold Exposure on the Content of Uncoupling Protein of BAT in Tupaia belangeri

作　　者：谢静[1] 王政昆[1] 张武先[1] 朱莉萍[1] 

机构地区：[1]云南师范大学生命科学学院教育部生物能源持续开发利用工程研究中心,昆明650092

出　　处：《动物学杂志》2008年第4期34-40,共7页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30560026);云南省科技强省研究重点项目(No.2007C000Z1)

摘　　要：自备抗血清采用酶联免疫法测定了中缅树鼩(Tupaia belangeri)在(5±1)℃冷暴露0 d、7 d、14 d、21d、28 d时,褐色脂肪组织(BAT)中解偶联蛋白1(UCP1)的含量。结果表明,随着冷暴露时间的延长,中缅树鼩的体重、褐色脂肪组织重量均表现出了增加的趋势,BAT线粒体总蛋白和UCP1的含量也呈增加的趋势,其中UCP1的含量在28 d时达到极显著水平,比对照组增加了55.9%。说明冷暴露能够诱导中缅树鼩UCP1表达增加,从而使其适应性产热增加。Uncoupling proteins （UCPs） increase proton leakage across the inner mitochondrial membrane. UCP1 in brown adipose tissue dissipates proton motive force as heat. This mechanism of nonshivering thermogenesis is considered as a monophyletic trait of endothermic placental mammals and provided a crucial advantage for life in the cold. Thereby, UCP1 is thus the only protein capable of mediating cold-acclimation-recruited adaptive adrenergic nonshivering thermogenesis. In this study, the content of UCP1 in BAT in Tree Shrews （ Tupaia belangeri ） was measured during cold exposure for 0 day, 7 days, 21 days and 28 days by enzyme-linked immunosorbent assay. The body weight, BAT weight, mitochondrial protein contents and UCP1 contents greatly increased with the cold exposure. The UCP1 content increased by 55.9 % （ P 〈 O. O1 ） significantly after 28 days cold acclimation. The changes in BAT thermogenic properties are important for the survival under cold stress in T. belangeri.

关 键 词：中缅树鼩 冷暴露 解偶联蛋白-1 酶联免疫 

分 类 号：Q4[生物学—生理学]