黑麂睾丸及精子的形态学观察  

Morphology of Testis and Spermatozoa in Muntiacus crinifrons

作　　者：孟祥辉[1] 陶勇[1] 张志忠 易刚 王根红 周世贤[1] 丁建平[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]合肥野生动物园,合肥230061

出　　处：《动物学杂志》2008年第4期122-126,共5页Chinese Journal of Zoology

基　　金：安徽省优秀青年科技基金项目(No.06041081)

摘　　要：研究了成年雄性黑麂(Muntiacus crinifrons)的睾丸和附睾尾精子形态。Gimsa染色后在显微镜下观察了黑麂精子的顶体形态,统计了顶体畸形和原生质滴存在情况,并与成年黄淮山羊(Capra hircus)的睾丸进行了对比。结果发现,黑麂睾丸长轴4.25 cm,短轴2.05 cm,明显低于成年黄淮山羊睾丸。黑麂精子长54.80μm,顶体呈圆柱形,顶体约覆盖精子头部的2/3,这个比例明显高于黄淮山羊。黑麂精子畸形率为21.50%,附睾尾部的精子活力为0.20,附睾尾精子原生质滴率为30.17%,顶体异常率为30.50%,与黄淮山羊基本接近。本实验为进一步研究黑麂的繁殖性能和保护提供了参考。Black Muntjac （Muntiacus crinifrons） is a critically endangered mammalian species. For the first time, this study investigated the testis and spermatozoa morphology of M. crinifrons by using Gimsa staining. The parameters of aerosome abnormality were recorded and compared with those of Huanghuai Goat. The results showed that the long axis of Black Muntjac testicle was 4.25 cm while the short axis was 2.05 cm, obviously lower than the values of Huanghuai Goat testicle. The length of Black Muntjac spermatozoon was 54.80 t^m and its acrosome was in a cylinder shape. The aerosome covered approximately 2/3 sperm head, while Huanghuai Goat acrosome covered only 1/2 sperm head. Furthermore,we found that the percentage of teratosperm in epididymal caudae was 21.50% ± 4.23%, and the spermatozoa mobility was 0.20. The percentage of sperm with protoplasmic droplet and abnormal acrosome was 30.17% ±4.45% and 30.50% ± 3.99%, respectively. These data will be useful for assisted reproduction and protection of this endangered species.

关 键 词：黑麂 精子 顶体 活力 

分 类 号：Q954[生物学—动物学]