人巨细胞病毒UL150基因在临床低传代分离株中的多态性研究  

Polymorphism analysis of human cytomegalovirus UL150 gene in low passage clinical isolates

作　　者：吉耀华[1] 阮强[1] 何蓉[1] 齐莹[1] 马艳萍[1] 孙峥嵘[1] 刘庆[1] 王继东[1] 

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《中华实验和临床病毒学杂志》2008年第4期281-283,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家自然科学基金资助项目（30170986）

摘　　要：目的研究人巨细胞病毒（human cvtomegalovirus，HCMV）UL150序列在临床低传代分离株中的多态性，探讨HCMV基因多态性与其感染引起不同临床症状之间的关系。方法对29株经荧光定量PCR方法（Q-PER）检测HCMV-DNA为阳性的临床低传代分离株进行HCMV UL150全序列PER扩增，并对PER扩增产物进行序列测定及分析。结果在29株临床低传代分离株中25株PCR扩增阳性，其中18株完成了测序。18株临床分离株HCMV UL150开放阅读框架（open reading frame，ORF）与HCMV Toledo株相应序列进行比较分析，显示18株低传代临床分离株的HCMVUL150 ORF均为1920bp，编码蛋白含有640个氨基酸，临床株的ORF及编码的氨基酸序列的长度均与Merlin株一致。结论HCMVUL150基因在临床分离株中存在着高度的多态性，未发现其与HCMV感染不同临床症状间存在明显的关系。Objective To investigate the polymorphism of human cytomegalovirus UL150 gene in low passage clinical isolates and try to study the relationship between the polymorphism and different pathogenesis of congenital HCMV infection. Methods PCR was performed to amplify the entire HCMV UL150 gene region of 29 clinical isolates, which had been proven containing detectable HCMV-DNA by using FQ-PCR. PCR amplification products were sequenced directly and the data were analysed. Results 25 among 29 isolates were amplified and 18 isolates were sequenced successfully. By comparison with the sequence of Toledo and Merlin, the length of UL150 ORFs in all 18 clinical isolates was similar to that of Merlin than Toledo. Conclusion HCMV UL150 DNA and deduced amino acid sequences is hypervariability.

关 键 词：巨细胞病毒 基因 UL150 

分 类 号：R373[医药卫生—病原生物学] R541.4[医药卫生—基础医学]