HIV／AIDS患者外周血T淋巴细胞亚群的研究  

Study on T cell subsets in HIV/AIDS patients

作　　者：李海英[1] 曹振环[1] 计云霞[1] 吴昊[1] 陈新月[1] 

机构地区：[1]首都医科大学附属北京佑安医院,100069

出　　处：《中华实验和临床病毒学杂志》2008年第4期284-286,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：北京市自然科学基金资助项目（5062019）;北京市科委艾滋病重大项目基金赞助（D0906003040591）;国家高技术研究发展计划（863计划）（2006AA02Z411）

摘　　要：目的通过对H／V／AIDS患者外周血T淋巴细胞亚群进行分析，探讨初始、记忆和效应T细胞各亚群的变化情况及其与疾病进展的关系。方法应用流式细胞仪检测15例正常人，79例HIWAIDS患者CD4〈200组17例、200≤CD4≤500组45例和CIM〉500组17例。外周血淋巴细胞中T细胞各亚群绝对数及百分比。结果随着疾病进展，CD4^＋初始细胞（Naive）计数和比例均逐渐减少（P〈0．001）；CD4^＋中枢性记忆T细胞（Tcm）计数逐渐降低（P〈0．001），但百分比逐渐升高（P〈0．002）；CD4^＋效应记忆性T细胞（TEM）百分比上升（P〈0．001）；CD8^＋Naive细胞计数及百分比均逐渐下降（P〈0．05）；CD8^＋TCM、TEM和终末分化的效应性记忆T细胞（TEMRA）的计数及百分比，各组间差异均无统计学意义（P〉0．05）。结论HIV感染者外周血T淋巴细胞亚群发生显著变化，幼稚型T淋巴细胞数目逐渐减少，功能型T淋巴细胞数目增加。本研究有助于对HIV致病机制的研究及疾病进展的监测。Objective Analyze Naive and Merrnory T cell subsets in HIV/AIDS patients and investigate their relationship with disease development. Methods T cell subsets from 15 normal control subjects, 79 HIV/ AIDS patients were detected by FCM. Results With diesase progression, CD4 ^＋ Naive cell counts and ratio was both decreased obviously（ P 〈 0.001 ） ; CD4 ＋ Tcm cell counts was significantly decreased （ P 〈 0.001 ）, CD4 ＋ Tcm cell ratio was obviously higher （ P = 0.002） ; CD4^＋ TEM cell ratio was obviously increased（ P 〈 0.001 ） ; CD8^＋ T Naive cell counts and ratio was also decreased obviously（ P 〈 0.05） ; CD8^＋ TCM , TEM, TEMRA are not significantly different.Conclusions The peripheral lymphocyte subsets in HIV/AIDS patients changed obviously. The counts of naive T cell decreased, while the proportion of memory T cell increased significantly. It will help to understand pathogenesis of HIV.

关 键 词：HIV 获得性免疫缺陷综合征 淋巴细胞亚群 

分 类 号：R512.91[医药卫生—内科学] R725.622.5[医药卫生—临床医学]