慢性HBV感染患者肝组织内CD4^＋CD25^＋调节性T淋巴细胞的表达  

Expression of intrahepatic CD4 ^＋ CD25^ ＋ regulatory T cells in patients with chronic hepatitis

作　　者：杨玉英[1] 郎振为[1] 兰孟东[1] 石晓红[1] 马沛卿[1] 沈冰[1] 王培之[1] 徐道振[1] 

机构地区：[1]北京地坛医院,100011

出　　处：《中华实验和临床病毒学杂志》2008年第4期296-298,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的观察慢性HBV感染患者免疫耐受期与免疫清除期肝组织中CD4^＋CD25^＋调节性T细胞的表达及分布情况。方法应用免疫组织化学法检测19例免疫耐受期及12例免疫清除期慢性乙型肝炎患者肝组织中FoxP3的表达，6例正常肝组织为对照。结果FoxP3阳性信号位于淋巴细胞胞核内，阳性细胞主要聚集在汇管区，肝窦内亦可见散在单个淋巴细胞呈阳性。在免疫耐受期及免疫清除期患者肝组织中FoxP3较正常肝组织明显增加（P〈0．01），免疫清除期患者肝组织内FoxP3明显高于免疫耐受期患者（P〈0．01）。免疫清除期患者肝组织中FoxP3阳性标记指数与ALT、HBeAg及HBV-DNA水平无明显相关性。免疫耐受期与免疫清除期两组相比，在年龄、ALT、TBIL、HBeAg、HBeAg及HBV-DNA水平方面差异均有统计学意义。结论肝组织中CD4^＋CD25^＋调节性T细胞在慢性HBV感染慢性化和抑制免疫，控制肝脏炎症反应方面可能起了重要作用。Objective To investigate the expression and distribution of intrahepatie CD4^＋ CD25^＋ regulatory T cells in immuno-tolerant and immuno-clearance phase of patients with chronic hepatitis B. Methods The expression of FoxP3 was detected in 19 cases of immuno-tolerant phase and 12 cases of immuno-clearance phase by immunohistochemistry. The relation between the intrahepatic expression of FoxP3 and the clinicopathological features were analyzed. Results The positive signal of FoxP3 is located in nuclear of lymphocyte and mainly aggregated in portal areas as well as occasionally scattered in hepatic sinusoids. The expression of intrahepatic FoxP3 in the group of immuno-tolerant phase was significantly increased than those in normal control （P 〈 0.01 ）, and greatly decreased than those in immuno-clearance phase （ P 〈 0.01 ）. No correlation was observed among the expression of intrahepatic FoxP3, ALT, levels of HBV DNA, HBeAg positive, in patients of immuno-clearance phase, respectively. There were significant differences between immuno-tolerant phase and immuno-clearance phase age, ALT, TBIL, PTA, HBV-DNA and detection of HBeAg but not in sex and family history of HBV infection. Conclusion CD4 ^＋ CD25^ ＋ regulatory T cells may play important roles in the clearance of HBV as well as in liver inflammation and iniurv during chronic HBV infection.

关 键 词：CD4淋巴细胞计数 CD25淋巴细胞计数 T淋巴细胞 肝炎 乙型 慢性 免疫 耐受 

分 类 号：R512.62[医药卫生—内科学]