N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸对转化生长因子β1和Smad7表达调节在大鼠硅肺纤维化形成过程中的作用  

Effect of AcSDKP on the expression of TGF-β1 and Smad7 in the lung of rats with silicosis

作　　者：李丹丹[1] 闫静波[1] 罗玲[1] 武鹍飞[1] 张丽娟[1] 王瑞敏[1] 杨嫣[2] 杨方[1] 

机构地区：[1]华北煤炭医学院实验研究中心,唐山063000 [2]北京大学医学部,临床03级北京100083

出　　处：《解剖学杂志》2008年第4期454-457,共4页Chinese Journal of Anatomy

基　　金：中华人民共和国人事部留学人员科技活动基金(国人厅发[2006]164号);河北省自然科学基金(C2005000807);唐山市新药基础研究重点实验室项目(04362001B-9)

摘　　要：目的:探讨N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸(AcSDKP)对大鼠硅肺纤维化模型肺组织中胶原含量、转化生长因子β1(TGFβ-1)和Smad 7表达的影响。方法:选用非暴露式气管灌注法制作大鼠硅肺模型,并给予AcSDKP。采用H-E染色进行形态学观察,胶原Van Gison染色观察硅肺结节与间质纤维化的形成及改变,羟脯氨酸法检测肺内总胶原含量,免疫组织化学法、免疫印迹法检测肺组织内TGFβ-1、Smad7蛋白的表达。结果:染尘大鼠肺内硅结节形成明显,硅肺模型制作成功。AcSDKP治疗组肺组织胶原含量低于硅肺模型组。与对照组相比,模型组大鼠肺组织内TGF-β1蛋白表达增加,Smad7蛋白表达降低;与模型组相比,给予AcSDKP后,大鼠肺组织内TGFβ-1蛋白表达明显降低,Smad7蛋白表达增高。结论:AcSDKP可能通过增加大鼠肺组织中Smad7蛋白的表达来抑制TGFβ-1信号转导,从而发挥抗硅肺纤维化的作用。Objective： To investigate the effect of N-acetyl seryl-aspartyl-lysyl-proline （AcSDKP） on collagen content and ex pressions of TGF -β1 and Smad7 in the lung of rats with silicosis. Methods： Rats were intratracheally instilled with silica as silicotic models and administrated AcSDKP in the experiment. Morphology was observed by HE staining. Siliconic nodule was detected by Van Gison staining. Collagen content was detected by hydroxyproline assay. The expressions of TGF-β1 and Smad7 were detected with immunohistochemistry and Western blot. Results ： The animal model of silicosis was successfully established by HE staining observation. The content of collagen in AcSDKP treated group was lower than that in silicotic model group. Compared with the control group, the expression of TGF -β1 increased in the lung tissue of the silicotic model, but that of Smad7 decreased. Compared with the model group, the expression of TGF-β1 reduced and that of Smad7 increased in the lung tissue in AcSDKP-treated group. Conclusion ： AcSDKP could inhibit TGF-β1 pathway by increasing the expression of Smad7 in the lung tissue, which may play a role in the anti-silicosis.

关 键 词：N-乙酰基-丝氨酰-天门冬酰-赖氨酰-脯氨酸 转化生长因子Β1 SMAD7 硅肺 

分 类 号：R563[医药卫生—呼吸系统]