去甲肾上腺素促进血管平滑肌增殖和细胞表型转化  

Influence of noradrenalin on proliferation and phenotype of vascular smooth muscle cells

作　　者：刘斌[1] 焦磊[1] 徐红涛[1] 张艳林[1] 王旻晨[1] 杨亚安[1] 陈尔齐[1] 吴开云[1] 

机构地区：[1]苏州大学医学院解剖学教研室,苏州215123

出　　处：《解剖学杂志》2008年第4期493-495,525,共4页Chinese Journal of Anatomy

基　　金：苏州大学创新团队项目(9034602);苏州大学医学发展基金(EE134519)

摘　　要：目的:研究去甲肾上腺素(NE)对血管平滑肌细胞(VSMC)表型转化和增殖的影响及作用机制。方法:用NE分别作用体外培养和血清饥饿的血管平滑肌细胞,用5-BrdU标记VSMC的增殖、RT-PCR检测VSMC表型标志基因SM22α和增殖负性调控基因高血压相关基因-1(HRG-1)的表达。结果:NE和OX-LDL分别作用血清培养的VSMC后,SM22α和HRG-1表达下调,5-BrdU标记的阳性增殖细胞增多;NE分别与α1-肾上腺素受体拮抗剂(α1-R-)和β1-肾上腺素受体拮抗剂(β1-R-)共作用时,SM22α和HRG-1的表达明显上调。而当NE作用血清饥饿VSMC时,NE上调SM22α和HRG-1的表达。结论:NE对VSMC有促表型转化和增殖作用,且这种作用呈现像神经样的可逆性调节作用。其作用机制主要通过肾上腺素受体α1和β1来实现的。Objective.. To study influence of NE on proliferation and phenotype switch of vascular smooth muscle cells （VSMC） and its mechanism. ,Methods ： The VSMC were cultured in vitro, incubated in bovine serum culture medium and serum-free medium, and then treated by NE. Proliferative SMC labeled by bromodeoxyuridine （Brdu） were detected with immunocytochemistry, and hypertension-related gene-1 （HRG-1） and smooth muscle 22α （SM22α） mRNA expressions were detected by RT-PCR. Results： HRG-1 and SM22α mRAN expressions were decreased in VSMCs treated by NE and OX- LDL, which were incubated in bovine serum culture medium and a lot of proliferative cells labeled by Brdu were observed. However, HRG-1 and SM22α mRNA expressions were up-regulated in VSMC cultured in serum-free medium,which was treated by NE. While VSMC cultured with NE receptor blockade （α1-R- and VSMC β1-R ）, HRG-1 and SM22α expressions were increased. Conclusion： These results show that the effect of NE on proliferation and phenotsrpe switch of VSMC is reversible This effect may regulate the vascular sympathetic nerve, and its mechanism may be accomplished via al and β1 receptor.

关 键 词：去甲肾上腺素 表型转化 血管平滑肌细胞 平滑肌22α 高血压相关基因-1 

分 类 号：R33[医药卫生—人体生理学]