混合脐血血浆对植物血凝集素诱导的脐血源性T淋巴细胞增殖、分化的影响  

Effects of mixed cord blood plasma on proliferation and differentiation of PHA-induced cord blood-derived T lymphocyte

作　　者：耿微[1] 张福琴[1] 张圣明[1] 张云[2] 周英凡[2] 

机构地区：[1]潍坊医学院组织学与胚胎学教研室,潍坊261042 [2]济宁市精神病防治院二病房,济宁272051

出　　处：《解剖学杂志》2008年第4期515-518,共4页Chinese Journal of Anatomy

摘　　要：目的:探讨混合脐血血浆(CBP)在植物血凝集素(PHA)诱导脐血源性T淋巴细胞中的作用。方法:收集脐血血浆和脐血单个核细胞;将脐血单个核细胞分为CBP+PHA和PHA两组,检测两组细胞增殖率及免疫表型。结果:CBP+PHA组细胞增殖指数低于PHA组,但无统计学意义;两组细胞表型都以CD3+、CD4+为主,两组差异无统计学意义。结论:混合脐血血浆对脐血源性T淋巴细胞增殖、分化影响不明显,可能与RPMI1640培养体系具有相似的效果。Objective： To explore the effects of mixed cord blood plasma （CBP） on phytohemagglutin （PHA） induction of cord blood-derived T cells. Methods： Cord blood plasma and umbilical cord blood mononuclear cells （CBMCs） were collected; CBMCs were divided two groups： CBP＋ PHA group and PHA group, and the proliferation index and immune pheno- types of each group were detected. Results： The proliferation index of CBP＋PHA group was lower than that in the PHA group without significant difference （P〉0. 05） ; the immune phenotypes of two groups were both mainly CD3- and CD4 without significant difference （P〉0. 05）. Conclusion： The effects of CBP on proliferation and differentiation of cord bloodderived T cells were similar to those of 20% RPMI1640.

关 键 词：脐血血浆 T淋巴细胞 增殖 分化 

分 类 号：R392[医药卫生—免疫学]